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dc.contributor.authorVovlas, Nicolaes_ES
dc.contributor.authorVovlas, Alessioes_ES
dc.contributor.authorLeonetti, Paolaes_ES
dc.contributor.authorLiébanas, Graciaes_ES
dc.contributor.authorCastillo, Pabloes_ES
dc.contributor.authorSubbotin, Sergei A.es_ES
dc.contributor.authorPalomares Rius, Juan E.es_ES
dc.identifier.citationEuropean Journal of Plant Pathology 143(4): 833-845 (2015)es_ES
dc.description.abstractThis research carried out an accurate identification of the root-knot and cyst-forming nematode species parasitizing white clover at the Laceno Lake area in Southern Italy. Two species, the root-knot nematode Meloidogyne hapla and the cyst nematode Heterodera daverti were identified by integrative taxonomic approaches (classical, isozyme pattern, and D2-D3 expansion segments of 28S rRNA, ITS rRNA and coxI of mtDNA gene sequences) and found parasitizing white clover roots. These nematodes were detected in stunted plants with a reduced number of rhizobium nodules and the host suitability was confirmed by the high nematode population densities ranging from 53 to 2350 eggs and J2s per g of fresh roots for M. hapla, and 1.36 eggs and J2s/cm3 of soil for H. daverti; and cyst nematode females were also detected on the roots of clover. The studies on the host-parasite relationships of nematode-feeding sites in white clover roots infected by these nematodes showed a high susceptible response. Meloidogyne hapla and H. daverti infections were also observed on nitrogen-fixing root nodules of white clover, where well established feeding sites allowed active nematode reproduction. Histological examination of nitrogen-fixing root nodule tissues revealed that the nematodes established their permanent feeding sites in the vascular bundles of nodules which appeared enlarged deformed and disorganised by the expansion of nematode feeding cells (giant cells and syncytium) and hyperplasia of the nodule cortex. Additionally, coxI of mtDNA gene is an efficient barcoding sequence for discriminating the identification of H. daverti from H. trifolii.es_ES
dc.description.sponsorshipThe present work has been done in the frame of the Apulia Region Programme: Misura 214/4 sub azione a “Progetti integrati per la Biodiversità” PSR 2007–2013, ASSE II “Miglioramento dell’ ambiente e dello Spazio Rurale” SaVeGraINPuglia.es_ES
dc.subjectClover pasturees_ES
dc.subjectcoxI genees_ES
dc.subjectHeterodera daverties_ES
dc.subjectMeloidogyne haplaes_ES
dc.subjectMolecular phylogenyes_ES
dc.subjectNitrogen fixation losseses_ES
dc.subjectrRNA genees_ES
dc.titleParasitism effects on white clover by root-knot and cyst nematodes and molecular separation of Heterodera daverti from H. trifoliies_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderRegione Pugliaes_ES
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