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Inflammatory up-regulation in the pathogenesis of calcific aortic valve stenosis

AuthorsParra Izquierdo, Iván; Castaños-Mollor, Irene; López, Javier ; Gómez, Cristina ; Ayuso, Sergio; San Román, José Alberto; Sánchez Crespo, Mariano; García-Rodríguez, Carmen
Issue Date2016
CitationXXXIX Congreso SEBBM (2016)
Abstract[Background and aim]: Calcific aortic valve stenosis (CAVS), the most prevalent valvulopathy in Western countries, is characterized by valve thickening. Many evidences point to inflammation as a key event for the development of fibrosis and valve calcification. Since interferons (IFN) coordinate a diverse array of cellular programs through transcriptional regulation of immunologically relevant genes, the aim of this study was to elucidate their role in the inflammatory process in human valve cells. [Materials and methods]: Endothelial and interstitial valve cells were isolated from stenotic aortic valve (obtained from valve replacement), and non-stenotic aortic and pulmonary valves (obtained from heart transplant recipients). After exposure to recombinant type I and II IFN, the expression of pro-inflammatory molecules was addressed by Western blot and flow cytometry, and monocyte adhesion assayed. [Results]: Experiments showed a blatant array of cell-specific responses to IFN, including the expression of COX-2, ICAM-1 and E-selectin in endothelial valve cells. Notably, the response was higher in aortic than in pulmonary valve cells. IFN also promoted monocyte adhesion to endothelial monolayers. In control/stenotic interstitial cells, IFNγstrongly induced ICAM-1; in addition, stenotic cells were responsive to IFNα. Strikingly, IFN strongly cooperated with LPS (TLR4 ligand) and Pam2CSK4 (TLR2/6 ligand) to induce COX-2 and ICAM-1 expression, being the synergistic effect higher in aortic than in pulmonary valve cells. [Conclusions]: IFN are more potent inducers of inflammation in aortic than pulmonary valve cells, thus correlating with clinical differences since pulmonary valve rarely suffer CAVS. Data open the way to explore IFN as a potential therapeutic target for CAVS.
DescriptionResumen del póster presentado al XXXIX Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca del 5 al 8 de septiembre de 2016.
Appears in Collections:(IBGM) Comunicaciones congresos
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