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dc.contributor.authorLevin, Elenaes_ES
dc.contributor.authorBallester Frutos, Ana Rosaes_ES
dc.contributor.authorRaphael, Ginates_ES
dc.contributor.authorFeigenberg, Oleges_ES
dc.contributor.authorLiu, Yongshenges_ES
dc.contributor.authorNorelli, Johnes_ES
dc.contributor.authorGonzález-Candelas, Luises_ES
dc.contributor.authorMao, Jinges_ES
dc.contributor.authorDardick, Christopheres_ES
dc.contributor.authorWisniewski, Michaeles_ES
dc.contributor.authorDroby, Samires_ES
dc.identifier.citationPLoS ONE 12(10): e0186023 (2017)es_ES
dc.description.abstractP. expansum is regarded as one of the most important postharvest rots of apple fruit and is also of great concern to fruit processing industries. Elucidating the pathogenicity mechanism of this pathogen is of utmost importance for the development of effective and safe management strategies. Although, many studies on modification of the host environment by the pathogen were done, its interactions with fruit during the early stages of infection and the virulence factors that mediate pathogenicity have not been fully defined. Effectors carrying LysM domain have been identified in numerous pathogenic fungi and their role in the first stages of infection has been established. In this study, we identified 18 LysM genes in the P. expansum genome. Amino acid sequence analysis indicated that P. expansum LysM proteins belong to a clade of fungal-specific LysM. Eleven of the discovered LysM genes were found to have secretory pathway signal peptide, among them, 4 (PeLysM1 PeLysM2, PeLysM3 and PeLysM4) were found to be highly expressed during the infection and development of decay of apple fruit. Effect of targeted deletion of the four putative PeLysM effectors on the growth and pathogenicity was studied. Possible interactions of PeLysM with host proteins was investigated using the yeast-two-hybrid system.es_ES
dc.description.sponsorshipIsrael Binational Agricultural Research and Development Fund (BARD), US-4774-14C, Prof. Samir Droby and Dr. Michael Wisniewski; Israel Science Foundation (ISF) and National Natural Science Foundation of China (NSFC), 1936/14, Prof. Samir Droby and Prof. Yongsheng Liu.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectMultiple sequence alignmentes_ES
dc.subjectChitin-triggered immunityes_ES
dc.subjectRice blast diseasees_ES
dc.subjectPathogenic fungies_ES
dc.titleIdentification and characterization of LysM effectors in Penicillium expansumes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderUnited States - Israel Binational Agricultural Research and Development Fundes_ES
dc.contributor.funderIsrael Science Foundationes_ES
dc.contributor.funderNational Natural Science Foundation of Chinaes_ES
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