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Stem cell therapy for corneal epithelium regeneration following good manufacturing and clinical procedures

AuthorsRamírez, Beatriz, E.; Sánchez, Ana; Herreras, José M.; Fernández, Itziar; García-Sancho, Javier ; Nieto-Miguel, Teresa; Calonge, Margarita
Issue Date2015
PublisherHindawi Publishing Corporation
CitationBioMed Research International 2015: 408495 (2015)
Abstract[Objective]: To evaluate outcomes of cultivated limbal epithelial transplantation (CLET) for management of ocular surface failure due to limbal stem cell deficiency (LSCD). [Design]: Prospective, noncomparative, interventional case series and extensive comparison with recent similar studies. [Participants]: Twenty eyes with LSCD underwent CLET (11 autologous; 9 allogeneic) and were followed up for 3 years. Etiologies were divided into 3 prognostic categories: Group 1, chemical injuries (7 eyes); Group 2, immune-based inflammation (4 eyes); and Group 3, noninflammatory diseases (9 eyes). [Intervention]: Autologous and allogeneic limbal epithelial cells were cultivated on amniotic membranes and transplanted. Evaluations were based on clinical parameters, survival analysis, and in vivo confocal microscopy (IVCM). European Union Tissues/Cells Directive and good manufacturing procedures were followed. [Main Outcome Measures]: Improved clinical parameters, absence of epithelial defects, and improved central corneal epithelial phenotype. [Results]: Success rate was 80% at 1-2 years and 75% at 3 years. Autografts and allografts had similar survival. Success rate was significantly lower in prognostic Group 1 (42.9%) than in Groups 2-3 (100% each). All clinical parameters improved substantially. By IVCM, 80% of cases improved in epithelial status. [Conclusions]: CLET improved corneal epithelium quality, with subsequent improvement in symptoms, quality of life, and vision. These results confirm that CLET is a valid therapy for ocular surface failure.
Publisher version (URL)http://dx.doi.org/10.1155/2015/408495
Identifiersdoi: 10.1155/2015/408495
e-issn: 2314-6141
issn: 2314-6133
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