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dc.contributor.authorSánchez Torres, Palomaes_ES
dc.contributor.authorVilanova, Lauraes_ES
dc.contributor.authorBallester Frutos, Ana Rosaes_ES
dc.contributor.authorLópez Pérez, Marioes_ES
dc.contributor.authorTeixidó, Neuses_ES
dc.contributor.authorViñas, Inmaculadaes_ES
dc.contributor.authorUsall, Josepes_ES
dc.contributor.authorGonzález-Candelas, Luises_ES
dc.contributor.authorTorres, Rosarioes_ES
dc.date.accessioned2017-10-30T12:02:42Z-
dc.date.available2017-10-30T12:02:42Z-
dc.date.issued2017-08-12-
dc.identifier.citationFood Microbiology 69: 123-135 (2018)es_ES
dc.identifier.issn0740-0020-
dc.identifier.urihttp://hdl.handle.net/10261/156889-
dc.description.abstractBlue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 °C. The ΔPeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the ΔPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in ΔPeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction.es_ES
dc.description.sponsorshipAuthors are grateful to the Spanish Government for its financial support with the projects AGL2008-04828-C03-03, AGL2011-30519-C03-01, AGL2011-30519-C03-02 and AGL2011-30519-CO3-03 from the “Ministerio de Economía y Competitividad” (MINECO, Spain), the CERCA Programme/Generalitat de Catalunya and Generalitat Valenciana (PROMETEOII/2014/027).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectApplees_ES
dc.subjectConidiationes_ES
dc.subjectFruit-fungal interactiones_ES
dc.subjectGene expressiones_ES
dc.subjectTranscription factores_ES
dc.subjectVirulencees_ES
dc.titleUnravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infectiones_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.fm.2017.08.005-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.fm.2017.08.005es_ES
dc.embargo.terms2018-08-12es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.contributor.funderGeneralitat de Catalunyaes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002809es_ES
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