English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/156621
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Analysis of the genetic diversity and structure of the Spanish apple genetic resources suggests the existence of an Iberian genepool

AuthorsPereira-Lorenzo, J.; Urrestarazu, J.; Ramos-Cabrer, A. M.; Miranda, C.; Pina, A.; Dapena, E.; Moreno Sánchez, María Ángeles ; Errea, Pilar; Llamero, N.; Díaz-Hernández, M. B.; Santesteban, L. G.; Laquidain, M. J.; Gogorcena Aoiz, Yolanda ; Urbina, V.; Dalmases, J.; Ascasíbar-Errasti, J; Royo, J. B.
KeywordsMalus × domestica Borkh
differentiation
SSR markers
population structure
variability
Issue DateNov-2017
PublisherJohn Wiley & Sons
CitationPereira-Lorenzo J, Urrestarazu J, Ramos-Cabrer AM, Miranda C, Pina A, Dapena E, Moreno MA, Errea P, Llamero N, Díaz-Hernández MB, Santesteban LG, Laquidain MJ, Gogorcena Y, Urbina V, Dalmases J, Ascasíbar-Errasti J, Royo JB. Analysis of the genetic diversity and structure of the Spanish apple genetic resources suggests the existence of an Iberian genepool. Annals of Applied Biology 171 (3): 424–440 (2017)
AbstractThe nature and structure of genetic diversity in the Spanish apple germplasm preserved at the national level was widely unknown, since studies performed to date on this topic have been exclusively carried out at the regional scale. Here, 1453 accessions from Spanish collections of Malus × domestica were evaluated with a common set of 13 SSR (Simple Sequence Repeats) markers in order to estimate genetic diversity, to identify the underlying genetic structure and to unravel the relationships among them and among a wide set of international cultivars for reference. In total, 737 unique genotypes were identified, 581 diploids and 156 triploids. Using a model-based Bayesian clustering procedure, two reconstructed populations were obtained for diploid genotypes; one retaining only Spanish cultivars (42% of genotypes), and a second containing all foreign cultivars the latter exhibiting evidence supporting the existence of a secondary sub-structure. Similarly, analysis performed on the 156 triploid genotypes also revealed two reconstructed populations; one exclusively associated with local Spanish genotypes (44%). The Jaccard coefficient allowed clustering by UPGMA (Unweighted Pair Group Method) diploid and triploid genotypes, and remarkable differences in allelic composition among the different partitioning levels were found. AMOVA analyses showed moderate but significant differentiation among the main groups (0.08 ≤ FST ≤ 0.12). Our results highlight an important fraction of the Spanish apple germplasm that constitutes a differentiated genepool with respect to the international and commercial apple cultivars. Moreover, the extent of the Spanish genetic diversity was spatially distributed along the northern Iberian Peninsula, suggesting an extensive migration of genotypes along the country. This study is the first valuable action for genetic conservation of apple at the national scale, and constitutes a decisive step towards the definition of a Spanish core collection that will be useful for further studies in dissecting the genetic control of important horticultural traits through genome-wide association analysis in apple.
Description87 Pags.- 3 Tabls.- 5 Figs.- 1 Suppl. Fig.- 5 Suppl. Tabls. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1744-7348
Publisher version (URL)http://dx.doi.org/10.1111/aab.12385
URIhttp://hdl.handle.net/10261/156621
DOI10.1111/aab.12385
ISSN0003-4746
E-ISSN1744-7348
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
GogorcenaY_AnnApplBiol_2017.pdf Embargoed until November 30, 20287,31 MBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.