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Título

Isolation and Characterization of a Lycopene ε-Cyclase Gene of Chlorella (Chromochloris) zofingiensis. Regulation of the Carotenogenic Pathway by Nitrogen and Light

AutorCordero, Baldo F. ; Couso, Inmaculada ; León, Rosa; Rodríguez, Herminia ; Vargas, M. Ángeles
Fecha de publicación21-sep-2012
EditorMultidisciplinary Digital Publishing Institute
CitaciónMarine Drugs 10 (9): 2069-2088 (2012)
ResumenThe isolation and characterization of the lycopene ε-cyclase gene from the green microalga <em>Chlorella </em>(<em>Chromochloris</em>) <em>zofingiensis</em> (<em>Czlcy-e</em>) was performed. This gene is involved in the formation of the carotenoids α-carotene and lutein. <em>Czlcy-e</em> gene encoded a polypeptide of 654 amino acids. A single copy of <em>Czlcy-e</em> was found in <em>C. zofingiensis</em>. Functional analysis by heterologous complementation in <em>Escherichia coli</em> showed the ability of this protein to convert lycopene to δ-carotene. In addition, the regulation of the carotenogenic pathway by light and nitrogen was also studied in <em>C. zofingiensis</em>. High irradiance stress did not increase mRNA levels of neither lycopene β<em>-</em>cyclase gene (<em>lcy-b</em>) nor lycopene ε-cyclase gene<em> </em>(<em>lcy-e</em>) as compared with low irradiance conditions, whereas the transcript levels of <em>psy</em>, <em>pds</em>, <em>chyB</em> and <em>bkt</em> genes were enhanced, nevertheless triggering the synthesis of the secondary carotenoids astaxanthin, canthaxanthin and zeaxanthin and decreasing the levels of the primary carotenoids α-carotene, lutein, violaxanthin and β-carotene. Nitrogen starvation <em>per se</em> enhanced mRNA levels of all genes considered, except <em>lcy-e and pds</em>, but did not trigger the synthesis of astaxanthin, canthaxanthin nor zeaxanthin. The combined effect of both high light and nitrogen starvation stresses enhanced significantly the accumulation of these carotenoids as well as the transcript levels of <em>bkt</em> gene, as compared with the effect of only high irradiance stress.
Versión del editorhttp://doi.org/10.3390/md10092069
URIhttp://hdl.handle.net/10261/155519
Identificadoresdoi: 10.3390/md10092069
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