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dc.contributor.authorMadrigal, Marvines_ES
dc.contributor.authorPla, Daviniaes_ES
dc.contributor.authorSanz, Libiaes_ES
dc.contributor.authorBarboza, Elexandraes_ES
dc.contributor.authorArroyo-Portilla, Cynthiaes_ES
dc.contributor.authorCorrêa-Netto, Carloses_ES
dc.contributor.authorGutiérrez, José Maríaes_ES
dc.contributor.authorAlape-Girón, Albertoes_ES
dc.contributor.authorFlores-Díaz, Mariettaes_ES
dc.contributor.authorCalvete, Juan J.es_ES
dc.identifier.citationPLoS Neglected Tropical Diseases 11(8):e0005793 (2017)es_ES
dc.description21 páginas, 8 figuras, 5 tablas.es_ES
dc.description.abstractBACKGROUND: Bothrops, Crotalus and Lachesis represent the most medically relevant genera of pitvipers in Central and South America. Similarity in venom phenotype and physiopathological profile of envenomings caused by the four nominal Lachesis species led us to hypothesize that an antivenom prepared against venom from any of them may exhibit paraspecificity against all the other congeneric taxa. METHODS: To assess this hypothesis, in this work we have applied antivenomics and immunochemical methods to investigate the immunoreactivity of three monovalent antivenoms and two polyvalent antivenoms towards the venoms from different geographic populations of three different Lachesis species. The ability of the antivenoms to neutralize the proteolytic, hemorrhagic, coagulant, and lethal activities of the seven Lachesis venoms was also investigated. RESULTS: A conspicuous pattern of immunorecognition and cross-neutralization for all effects was evident by the polyspecific antivenoms, indicating large immunoreactive epitope conservation across the genus during more than 10 million years since the Central and South American bushmasters diverged. CONCLUSIONS: Despite the broad geographic distribution of Lachesis, antivenoms against venoms of different species are effective in the neutralization of congeneric venoms not used in the immunization mixture, indicating that they can be used equivalently for the clinical treatment of any lachesic envenoming. GENERAL SIGNIFICANCE: This study demonstrates that antivenoms raised against venom of different Lachesis species are indistinctly effective in the neutralization of congeneric venoms not used in the immunization mixture, indicating that antivenoms against conspecific venoms may be used equivalently for the clinical treatment of envenomings caused by any bushmaster species.es_ES
dc.description.sponsorshipThis study received financial support from Vicerrectorı´a de Investigacio´n, University of Costa Rica (project 741-B4-098) (MED,AGG, MMV), and Ministerio de Economı´a y Competitividad, Madrid, Spain (grant BFU2013- 42833-P) (JJC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleCross-reactivity, antivenomics, and neutralization of toxic activities of Lachesis venoms by polyspecific and monospecific antivenomses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
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