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Título

Frq2 from Drosophila melanogaster: Cloning, expression, purification, crystallization and preliminary X-ray analysis

Autor Baños-Mateos, S.; Chaves Sanjuán, Antonio; Mansilla, A.; Ferrús, Alberto ; Sánchez-Barrena, M.J.
Palabras clave Drosophila melanogaster Frq2 Calcium-binding proteins
Fecha de publicación 2014
Citación Acta Crystallographica Section F:Structural Biology Communications 70: 530- 534 (2014)
ResumenDrosophila melanogaster contains two calcium-binding proteins, Frq1 and Frq2, in the nervous system that control the number of synapses and the probability of release. To understand the differential function of the two proteins, whose sequence is only 5% dissimilar, the crystal structures of Frq1 and Frq2 are needed. Here, the cloning, expression, purification, crystallization and preliminary crystallographic analysis of Frq2 are presented. The full-length protein was purified using a two-step chromatographic procedure. Two different diffracting crystal forms were obtained using a progressive streak-seeding method and detergents. © 2014 International Union of Crystallography All rights reserved.
URI http://hdl.handle.net/10261/155016
Identificadoresdoi: 10.1107/S2053230X14005408
issn: 2053-230X
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