English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/154981
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 5 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

Tobacco rattle virus 16K silencing suppressor binds ARGONAUTE 4 and inhibits formation of RNA silencing complexes

Otros títulosRNA silencing suppression by TRV 16K
Autor Fernández-Calvino, L. ; Martínez-Priego, Llucia ; Szabo, Edit Z.; Guzmán-Benito, Irene; González, Inmaculada; Canto, Tomás ; Lakatos, Lóránt; Llave, César
Fecha de publicación ene-2016
EditorMicrobiology Society
Citación J Gen Virol. 97(1):246-57 (2016)
ResumenThe cysteine-rich 16K protein of tobacco rattle virus (TRV), the type member of the genus Tobravirus, is known to suppress RNA silencing. However, the mechanism of action of the 16K suppressor is not well understood. In this study, we used a GFP-based sensor strategy and an Agrobacterium-mediated transient assay in Nicotiana benthamiana to show that 16K was unable to inhibit the activity of existing small interfering RNA (siRNA)- and microRNA (miRNA)-programmed RNA-induced silencing effector complexes (RISCs). In contrast, 16K efficiently interfered with de novo formation of miRNA- and siRNA-guided RISCs, thus preventing cleavage of target RNA. Interestingly, we found that transiently expressed endogenous miR399 and miR172 directed sequence-specific silencing of complementary sequences of viral origin. 16K failed to bind small RNAs, although it interacted with ARGONAUTE 4, as revealed by bimolecular fluorescence complementation and immunoprecipitation assays. Site-directed mutagenesis demonstrated that highly conserved cysteine residues within the N-terminal and central regions of the 16K protein are required for protein stability and/or RNA silencing suppression.
Descripción 35 p.-5 fig.1 tab. supl.
Versión del editorhttp://dx.doi.org/10.1099/jgv.0.000323
URI http://hdl.handle.net/10261/154981
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
JGV 2015.pdfPostprint632,47 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.