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DNA Barcoding of Iberian butterflies enables a continental-scale assessment of potential cryptic diversity

AuthorsDincă, Vlad ; Montagud, Sergio; Talavera, Gerard ; Hernández-Roldán, Juan L.; Munguira, Miguel L.; García-Barros, Enrique; Hebert, Paul D. N.; Vila, Roger
KeywordsCryptic species
DNA barcodes
Issue DateJul-2013
CitationXVIII European Congress of Lepidopterology (2013)
AbstractWhile the number of DNA barcoding studies has dramatically increased during the last years, large scale comprehensive surveys referring to the European butterflies are still lacking. Based on intensive sampling from various regions of Spain and Portugal, we have gathered ca. 3450 DNA barcodes for all Iberian butterfly species, with an average of ca. 15 specimens per species. Subsequently, we combined the Iberian dataset with the other published Barcode of Life Data Systems (BOLD) projects dealing with the European butterfly fauna (covering especially well Romania and southern Germany). This resulted in a dataset of 5782 sequences of carefully identified specimens corresponding to more than 60% of all European butterfly species (with an average of ca. 19 specimens per species). This large dataset, which includes a good representation of both Mediterranean and temperate areas, provides novel insights not only into the effectiveness of DNA barcoding to identify European butterflies, but also into the levels of potential butterfly cryptic diversity at a continental scale. The cases highlighted provide guidelines for future research and have numerous implications for taxonomical, ecological, biogeographical and conservation studies of butterflies. Finally, we will also introduce the next step in our assessment of butterfly genetic diversity: the DNA barcoding of West Mediterranean butterflies.
DescriptionTrabajo presentado en el XVIII European Congress of Lepidopterology, celebrado en Blagoevgrad (Bulgaria) del 29 de julio al 4 de agosto de 2013.
Appears in Collections:(IBE) Comunicaciones congresos
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