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Regulatory lymphocytes are key factors in mhc-independent resistance to EAE
|Authors:||Marín, N.; Mecha, Miriam ; Espejo, Carmen; Mestre, Leyre ; Eixarch, H.; Montalbán, Xavier; Alvarez-Cermeño, José C.; Guaza, Carmen ; Villar, L.M.|
|Citation:||Journal of Immunology Research 2014: 156380 (2014)|
|Abstract:||[Background and Objectives] Resistant and susceptible mouse strains to experimental autoimmune encephalomyelitis (EAE), an inducible demyelinating experimental disease serving as animal model for multiple sclerosis, have been described. We aimed to explore MHC-independent mechanisms inducing resistance to EAE.|
[Methods] For EAE induction, female C57BL/6 (susceptible strain) and CD1 (resistant outbred strain showing heterogeneous MHC antigens) mice were immunized with the 35-55 peptide of myelin oligodendrocyte glycoprotein (MOG35-55). We studied T cell proliferation, regulatory and effector cell subpopulations, intracellular and serum cytokine patterns, and titers of anti-MOG serum antibodies.
[Results] Upon immunization with MOG35-55, T lymphocytes from susceptible mice but not that of resistant strain were capable of proliferating when stimulated with MOG35-55. Accordingly, resistant mice experienced a rise in regulatory B cells (P = 0.001) and, to a lower extent, in regulatory T cells (P = 0.02) compared with C57BL/6 susceptible mice. As a consequence, MOG35-55-immunized C57BL/6 mice showed higher percentages of CD4+ T cells producing both IFN-gamma (P = 0.02) and IL-17 (P = 0.009) and higher serum levels of IL-17 (P = 0.04) than resistant mice.
[Conclusions] Expansion of regulatory B and T cells contributes to the induction of resistance to EAE by an MHC-independent mechanism. © 2014 Nieves Marín et al.
|Publisher version (URL):||http://doi.org/10.1155/2014/156380|
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