Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/154387
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | López-Villarejo, Juan | - |
dc.contributor.author | Lobato-Márquez, Damián | - |
dc.contributor.author | Díaz-Orejas, Ramón | - |
dc.date.accessioned | 2017-08-24T13:03:10Z | - |
dc.date.available | 2017-08-24T13:03:10Z | - |
dc.date.issued | 2015-02-05 | - |
dc.identifier | doi: 10.3390/toxins7020478 | - |
dc.identifier.citation | Toxins 7 (2): 478-492 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/154387 | - |
dc.description.abstract | <i>kis-kid</i>, the auxiliary maintenance system of plasmid R1 and <i>copB</i>, the auxiliary copy number control gene of this plasmid, contribute to increase plasmid replication efficiency in cells with lower than average copy number. It is thought that Kis antitoxin levels decrease in these cells and that this acts as the switch that activates the Kid toxin; activated Kid toxin reduces <i>copB</i>-mRNA levels and this increases RepA levels that increases plasmid copy number. In support of this model we now report that: (i) the Kis antitoxin levels do decrease in cells containing a mini-R1 plasmid carrying a <i>repA</i> mutation that reduces plasmid copy number; (ii) <i>kid</i>-dependent replication rescue is abolished in cells in which the Kis antitoxin levels or the CopB levels are increased. Unexpectedly we found that this coordination significantly increases both the copy number of the <i>repA</i> mutant and of the wt mini-R1 plasmid. This indicates that the coordination between plasmid replication functions and <i>kis-kid</i> system contributes significantly to control plasmid R1 replication. | - |
dc.description.sponsorship | This study was founded by projects CSD2008-00013 and BFU2011-25939 of the Spanish Ministry of Science and Innovation. Discussions related to this work with members of the group are kindly acknowledged. The comments and corrections to the manuscript of Elizabeth Diago-Navarro are kindly acknowledged. | - |
dc.description.sponsorship | We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). | - |
dc.publisher | Multidisciplinary Digital Publishing Institute | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication | - |
dc.type | artículo | - |
dc.identifier.doi | 10.3390/toxins7020478 | - |
dc.relation.publisherversion | https://doi.org/10.3390/toxins7020478 | - |
dc.date.updated | 2017-08-24T13:03:10Z | - |
dc.rights.license | (http://creativecommons.org/licenses/by/4.0/) | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | - |
dc.identifier.pmid | 25664511 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
toxins-07-00478.pdf | 845,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
5
checked on 22-abr-2024
SCOPUSTM
Citations
9
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
8
checked on 25-feb-2024
Page view(s)
280
checked on 24-abr-2024
Download(s)
214
checked on 24-abr-2024