English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/154387
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication

Autor López-Villarejo, Juan ; Lobato-Márquez, Damián ; Díaz-Orejas, Ramón
Fecha de publicación 5-feb-2015
EditorMultidisciplinary Digital Publishing Institute
Citación Toxins 7 (2): 478-492 (2015)
Resumen<i>kis-kid</i>, the auxiliary maintenance system of plasmid R1 and <i>copB</i>, the auxiliary copy number control gene of this plasmid, contribute to increase plasmid replication efficiency in cells with lower than average copy number. It is thought that Kis antitoxin levels decrease in these cells and that this acts as the switch that activates the Kid toxin; activated Kid toxin reduces <i>copB</i>-mRNA levels and this increases RepA levels that increases plasmid copy number. In support of this model we now report that: (i) the Kis antitoxin levels do decrease in cells containing a mini-R1 plasmid carrying a <i>repA</i> mutation that reduces plasmid copy number; (ii) <i>kid</i>-dependent replication rescue is abolished in cells in which the Kis antitoxin levels or the CopB levels are increased. Unexpectedly we found that this coordination significantly increases both the copy number of the <i>repA</i> mutant and of the wt mini-R1 plasmid. This indicates that the coordination between plasmid replication functions and <i>kis-kid</i> system contributes significantly to control plasmid R1 replication.
URI http://hdl.handle.net/10261/154387
Identificadoresdoi: 10.3390/toxins7020478
Aparece en las colecciones: Colección MDPI
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
toxins-07-00478.pdf845,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.