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http://hdl.handle.net/10261/153745
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Moreno-Beltrán, Blas | es_ES |
dc.contributor.author | Guerra-Castellano, Alejandra | es_ES |
dc.contributor.author | Díaz-Quintana, Antonio | es_ES |
dc.contributor.author | Conte, Rebecca del | es_ES |
dc.contributor.author | García-Mauriño, Sofía M. | es_ES |
dc.contributor.author | Díaz-Moreno, Sofía | es_ES |
dc.contributor.author | González-Arzola, Katiuska | es_ES |
dc.contributor.author | Santos-Ocaña, Carlos | es_ES |
dc.contributor.author | Velázquez-Campoy, Adrián | es_ES |
dc.contributor.author | Rosa, Miguel A. de la | es_ES |
dc.contributor.author | Turano, Paola | es_ES |
dc.contributor.author | Díaz-Moreno, Irene | es_ES |
dc.date.accessioned | 2017-08-01T08:03:56Z | - |
dc.date.available | 2017-08-01T08:03:56Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences 114(15): E3041–E3050 (2017) | es_ES |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10261/153745 | - |
dc.description.abstract | Regulation of mitochondrial activity allows cells to adapt to changing conditions and to control oxidative stress, and its dysfunction can lead to hypoxia-dependent pathologies such as ischemia and cancer. Although cytochrome c phosphorylation—in particular, at tyrosine 48—is a key modulator of mitochondrial signaling, its action and molecular basis remain unknown. Here we mimic phosphorylation of cytochrome c by replacing tyrosine 48 with p-carboxy-methyl-L-phenylalanine (pCMF). The NMR structure of the resulting mutant reveals significant conformational shifts and enhanced dynamics around pCMF that could explain changes observed in its functionality: The phosphomimetic mutation impairs cytochrome c diffusion between respiratory complexes, enhances hemeprotein peroxidase and reactive oxygen species scavenging activities, and hinders caspase-dependent apoptosis. Our findings provide a framework to further investigate the modulation of mitochondrial activity by phosphorylated cytochrome c and to develop novel therapeutic approaches based on its prosurvival effects. | es_ES |
dc.description.sponsorship | Financial support was provided by the Spanish Ministry of Economy and Competitiveness (Grants BFU2015-71017-P/BMC and BFU2015-19451/BMC, cofounded by FEDER EU), European Union (Bio-MR-00130 and CALIPSO-312284), Ramon Areces Foundation, and Andalusian Government (BIO198). B.M.-B. was awarded a PhD fellowship from the Spanish Ministry of Education (AP2009-4092) and a short-term traveling fellowship from the European Bio-NMR Project. A.G.-C. was awarded a PhD fellowship from the CSIC (JaePre-2011-01248). | - |
dc.language.iso | eng | es_ES |
dc.publisher | National Academy of Sciences (U.S.) | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-71017-P | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/312284 | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-19451-BMC | - |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Structural basis of mitochondrial dysfunction in response to cytochrome c phosphorylation at tyrosine 48 | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1073/pnas.1618008114 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | htpp://dx.doi.org/10.1073/pnas.1618008114 | es_ES |
dc.identifier.e-issn | 1091-6490 | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Ministerio de Educación (España) | - |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.identifier.pmid | 28348229 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Guerra-Castellano_et_al_PNAS_2017.pdf | 2,6 MB | Adobe PDF | Visualizar/Abrir |
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