Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/153127
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Confocal microscopy for astrocyte in vivo imaging: Recycle and reuse in microscopy |
Autor: | Pérez-Álvarez, Alberto CSIC ORCID; Araque, Alfonso CSIC ORCID; Martín, E.D. | Fecha de publicación: | 29-abr-2013 | Editor: | Frontiers Media | Citación: | Front. Cell. Neurosci. 7: 51 (2013) | Resumen: | In vivo imaging is one of the ultimate and fundamental approaches for the study of the brain. Two-photon laser scanning microscopy (2PLSM) constitutes the state-of-the-art technique in current neuroscience to address questions regarding brain cell structure, development and function, blood flow regulation and metabolism. This technique evolved from laser scanning confocal microscopy (LSCM), which impacted the field with a major improvement in image resolution of live tissues in the 1980s compared to widefield microscopy. While nowadays some of the unparalleled features of 2PLSM make it the tool of choice for brain studies in vivo, such as the possibility to image deep within a tissue, LSCM can still be useful in this matter. Here we discuss the validity and limitations of LSCM and provide a guide to perform high-resolution in vivo imaging of the brain of live rodents with minimal mechanical disruption employing LSCM. We describe the surgical procedure and experimental setup that allowed us to record intracellular calcium variations in astrocytes evoked by sensory stimulation, and to monitor intact neuronal dendritic spines and astrocytic processes as well as blood vessel dynamics. Therefore, in spite of certain limitations that need to be carefully considered, LSCM constitutes a useful, convenient, and affordable tool for brain studies in vivo. | Versión del editor: | http://dx.doi.org/10.3389/fncel.2013.00051 | URI: | http://hdl.handle.net/10261/153127 | DOI: | 10.3389/fncel.2013.00051 | ISSN: | 1662-5102 |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Confocal microscopy for astrocyte in vivo imaging.pdf | 771,69 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
14
checked on 06-abr-2024
SCOPUSTM
Citations
20
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 28-feb-2024
Page view(s)
243
checked on 22-abr-2024
Download(s)
230
checked on 22-abr-2024