English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/153046
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

AuthorsAlcántara de la Cruz, Ricardo; Rojano-Delgado, Antonia María; Giménez, María J. ; Cruz-Hipólito, Hugo Enrique; Domínguez Valenzuela, José A.; Barro Losada, Francisco ; Prado, R. del
Issue Date18-Nov-2016
PublisherFrontiers Media
CitationFrontiers in Plant Science 7: 1742 (2016)
AbstractLeptochloa virgata (L.) P. Beauv. is an annual weed common in citrus groves in the states of Puebla and Veracruz, Mexico limiting their production. Since 2010, several L. virgata populations were identified as being resistant to glyphosate, but studies of their resistance mechanisms developed by this species have been conducted. In this work, three glyphosate-resistant populations (R8, R14, and R15) collected in citrus orchards from Mexico, were used to study their resistance mechanisms comparing them to one susceptible population (S). Dose-response and shikimic acid accumulation assays confirmed the glyphosate resistance of the three resistant populations. Higher doses of up to 720 g ae ha-1 (field dose) were needed to control by 50% plants of resistant populations. The S population absorbed between 7 and 13% more 14C-glyphosate than resistant ones, and translocated up to 32.2% of 14C-glyphosate to the roots at 96 h after treatment (HAT). The R8, R14, and R15 populations translocated only 24.5, 26.5, and 21.9%, respectively. The enzyme activity of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was not different in the S, R8 and R14 populations. The R15 Population exhibited 165.9 times greater EPSPS activity. Additionally, this population showed a higher EPSPS basal activity and a substitution in the codon 106 from Proline to Serine in the EPSPS protein sequence. EPSPS gene expression in the R15 population was similar to that of S population. In conclusion, the three resistant L. virgata populations show reduced absorption and translocation of 14C-glyphosate. Moreover, a mutation and an enhanced EPSPS basal activity at target-site level confers higher resistance to glyphosate. These results describe for the first time the glyphosate resistance mechanisms developed by resistant L. virgata populations of citrus orchards from Mexico.
Publisher version (URL)http://dx.doi.org/10.3389/fpls.2016.01742
URIhttp://hdl.handle.net/10261/153046
DOI10.3389/fpls.2016.01742
ISSN1664-462X
Appears in Collections:(IAS) Artículos
Files in This Item:
File Description SizeFormat 
First Resistance Mechanisms Characterization.pdf1,62 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.