English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/152971
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

The Amino Acid Substitution Q65H in the 2C Protein of Swine Vesicular Disease Virus Confers Resistance to Golgi Disrupting Drugs

AutorVázquez-Calvo, Ángela; Caridi, Flavia; González-Magaldi, Mónica; Saiz, Juan Carlos; Sobrino Castelló, Francisco; Martín-Acebes, Miguel Ángel
Fecha de publicación27-abr-2016
EditorFrontiers Media
CitaciónFrontiers in Microbiology 7: 612 (2016)
ResumenSwine vesicular disease virus (SVDV) is a porcine pathogen and a member of the species Enterovirus B within the Picornaviridae family. Brefeldin A (BFA) is an inhibitor of guanine nucleotide exchange factors of Arf proteins that induces Golgi complex disassembly and alters the cellular secretory pathway. Since BFA has been shown to inhibit the RNA replication of different enteroviruses, including SVDV, we have analyzed the effect of BFA and of golgicide A (GCA), another Golgi disrupting drug, on SVDV multiplication. BFA and GCA similarly inhibited SVDV production. To investigate the molecular basis of the antiviral effect of BFA, SVDV mutants with increased resistance to BFA were isolated. A single amino acid substitution, Q65H, in the non-structural protein 2C was found to be responsible for increased resistance to BFA. These results provide new insight into the relationship of enteroviruses with the components of the secretory pathway and on the role of SVDV 2C protein in this process.
Versión del editorhttp://dx.doi.org/10.3389/fmicb.2016.00612
URIhttp://hdl.handle.net/10261/152971
DOI10.3389/fmicb.2016.00612
ISSN1664-302X
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
The Amino Acid Substitution Q65H.pdf5,1 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.