English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/152731
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorHerrera, Mercedes-
dc.contributor.authorPeña, Cristina-
dc.contributor.authorLarriba, María Jesús-
dc.contributor.authorBonilla, Félix-
dc.contributor.authorGarcía, Vanesa-
dc.date.accessioned2017-07-13T11:52:17Z-
dc.date.available2017-07-13T11:52:17Z-
dc.date.issued2015-
dc.identifier.citationV Encuentro Científico de Jóvenes Investigadores de la RTICC (2015)-
dc.identifier.urihttp://hdl.handle.net/10261/152731-
dc.descriptionResumen del trabajo presentado al V Encuentro Científico de Jóvenes Investigadores de la Red Temática de Investigación Cooperativa en Cáncer, celebrado en Pamplona, Navarrra el 5 de octubre de 2015.-- et al.-
dc.description.abstract[Purpose]: Tumor-derived exosomes are a significant mechanism of intercellular communication, affecting tumor-related pathways, such as angiogenesis, drug resistance and immune response. This study analyzed the release of exosomes from breast tumor cells with different capacities of stemness/metastasis based on CXCR4 expression and evaluated their involvement in the capacity of generate oncogenic features in recipient cells. [Experimental design]: Oncogenic effects of breast cancer cells-derived exosomes were evaluated in vitro and in vivo models. Exosome cargo in mRNAs involved in stem cell differentiation and metastasis were analyzed by specific quantitative PCR arrays. Finally, stemness- and metastatic-related mRNA most differentially detected in exosomes were analyzed in plasma of breast cancer patients assessing its capacity as a prognosis marker. [Results]: In vitro, exosomes released from CXCR4-tumor cells modify stemness markers, and proliferation, migration and invasion capacities of neighboring cells. Accordingly, inoculation of CXCR4-cells-derived exosomes in immunocompromised mice mediated primary tumor growth and metastatic potential. Exosomes isolated from CXCR4-cells and stemlike cells were highly enriched in stemness- and metastatic-related mRNAs and showed high homology between them. Furthermore, comparative analysis of mRNAs contained in exosomes isolated from patients revealed a gene signature of mRNAs highly enriched in exosomes of patients with worse prognosis. [Conclusions]: Our data support the view that exosomes released by CXCR4-cancer cells that exhibit stem-like properties are capable to transfer these oncogenic features to recipient cells. In addition, our results show the involvement of >stemness and metastatic signature> mRNA in exosomes from plasma in breast cancer patient survival.-
dc.rightsclosedAccess-
dc.titleTumor-derived exosomes enriched in stemness/metastatic-related mRNAs promote oncogenic potential in breast cancer. Specific prognostic exosome signature-
dc.typeComunicación de congreso-
dc.date.updated2017-07-13T11:52:19Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.relation.csic-
Appears in Collections:(IIBM) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show simple item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.