Tumor-derived exosomes enriched in stemness/metastatic-related mRNAs promote oncogenic potential in breast cancer. Specific prognostic exosome signature

Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/152731

COMPARTIR / EXPORTAR:

SHARE BASE	Comparte tu historia de Acceso Abierto
Visualizar otros formatos: MARC \| Dublin Core \| RDF \| ORE \| MODS \| METS \| DIDL \| DATACITE
Refman EndNote Bibtex RefWorks Excel CSV PDF DataCite Send via email

Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Herrera, Mercedes	-
dc.contributor.author	Peña, Cristina	-
dc.contributor.author	Larriba, María Jesús	-
dc.contributor.author	Bonilla, Félix	-
dc.contributor.author	García, Vanesa	-
dc.date.accessioned	2017-07-13T11:52:17Z	-
dc.date.available	2017-07-13T11:52:17Z	-
dc.date.issued	2015	-
dc.identifier.citation	V Encuentro Científico de Jóvenes Investigadores de la RTICC (2015)	-
dc.identifier.uri	http://hdl.handle.net/10261/152731	-
dc.description	Resumen del trabajo presentado al V Encuentro Científico de Jóvenes Investigadores de la Red Temática de Investigación Cooperativa en Cáncer, celebrado en Pamplona, Navarrra el 5 de octubre de 2015.-- et al.	-
dc.description.abstract	[Purpose]: Tumor-derived exosomes are a significant mechanism of intercellular communication, affecting tumor-related pathways, such as angiogenesis, drug resistance and immune response. This study analyzed the release of exosomes from breast tumor cells with different capacities of stemness/metastasis based on CXCR4 expression and evaluated their involvement in the capacity of generate oncogenic features in recipient cells. [Experimental design]: Oncogenic effects of breast cancer cells-derived exosomes were evaluated in vitro and in vivo models. Exosome cargo in mRNAs involved in stem cell differentiation and metastasis were analyzed by specific quantitative PCR arrays. Finally, stemness- and metastatic-related mRNA most differentially detected in exosomes were analyzed in plasma of breast cancer patients assessing its capacity as a prognosis marker. [Results]: In vitro, exosomes released from CXCR4-tumor cells modify stemness markers, and proliferation, migration and invasion capacities of neighboring cells. Accordingly, inoculation of CXCR4-cells-derived exosomes in immunocompromised mice mediated primary tumor growth and metastatic potential. Exosomes isolated from CXCR4-cells and stemlike cells were highly enriched in stemness- and metastatic-related mRNAs and showed high homology between them. Furthermore, comparative analysis of mRNAs contained in exosomes isolated from patients revealed a gene signature of mRNAs highly enriched in exosomes of patients with worse prognosis. [Conclusions]: Our data support the view that exosomes released by CXCR4-cancer cells that exhibit stem-like properties are capable to transfer these oncogenic features to recipient cells. In addition, our results show the involvement of >stemness and metastatic signature> mRNA in exosomes from plasma in breast cancer patient survival.	-
dc.rights	closedAccess	-
dc.title	Tumor-derived exosomes enriched in stemness/metastatic-related mRNAs promote oncogenic potential in breast cancer. Specific prognostic exosome signature	-
dc.type	comunicación de congreso	-
dc.date.updated	2017-07-13T11:52:19Z	-
dc.description.version	Peer Reviewed	-
dc.language.rfc3066	eng	-
dc.relation.csic	Sí	-
dc.type.coar	http://purl.org/coar/resource_type/c_5794	es_ES
item.fulltext	No Fulltext	-
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.openairetype	comunicación de congreso	-
item.cerifentitytype	Publications	-
item.grantfulltext	none	-
Aparece en las colecciones:	(IIBM) Comunicaciones congresos