High-resolution spatio-temporal distribution of a coastal phytoplankton bloom using laser in situ scattering and transmissometry (LISST)

Abstract

Development of optical observation technologies provides new insights into harmful algal bloom (HAB) detection and assessment of HAB species dynamics. Based on preliminary laboratory tests, a laser in situ scattering and transmissometry instrument (LISST-100X) was used to monitor a high-biomass phytoplankton proliferation in the field. Short-term spatial and temporal changes in particle size distribution were measured during a recurrent Alexandrium taylori outbreak. Since the bloom was not monospecific, a size-fraction method to discriminate particular species from LISST-100X measurements was proposed. The results were validated to simultaneous microscopic counts of phytoplankton, and the significantly positive correlation obtained between the two methodologies confirmed the instrument's ability to discriminate phytoplankton at the group and species level. The LISST-100X obtains high-resolution in situ data, and is therefore a better alternative than the traditional microscope for assessing temporal and spatial evolution of HABs. Field observations showed high variability over a short time scale associated with diel vertical migration of A. taylori and the whole phytoplankton population (nanoplankton and microplankton). A numerical circulation model was used to investigate the influence of beach hydrodynamics in the observed horizontal variability. Simulations of the model suggested an important role of daily coastal circulation in determining the distribution of A. taylori in coastal environments