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Título

Identification of the ligand of Pru p 3, a peach LTP

AutorCubells-Baeza, Nuria; Gómez-Casado, Cristina; Tordesillas, Leticia; Ramírez-Castillejo, Carmen; Garrido-Arandia, María; González-Melendi, Pablo ; Herrero Romero, María ; Pacios, Luis F.; Díaz-Perales, Araceli
Palabras clavePru p 3
Lipid transfer protein
Secondary metabolites
Camptothecin
Flower development
Pollination
Fruit development
Fecha de publicaciónmay-2017
EditorSpringer
CitaciónCubells-Baeza N, Gómez-Casado C, Tordesillas L, Ramírez-Castillejo C, Garrido-Arandia M, González-Melendi P, Herrero M, Pacios LF, Díaz-Perales A. Identification of the ligand of Pru p 3, a peach LTP. Plant Molecular Biology 94 (1-2): 33–44 (2017)
ResumenThe allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
Descripción12 Pags.- 7 Figs.
Versión del editorhttp://dx.doi.org/10.1007/s11103-017-0590-z
URIhttp://hdl.handle.net/10261/151814
DOI10.1007/s11103-017-0590-z
ISSN0167-4412
E-ISSN1573-5028
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