Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/151719
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | An optimised protocol to isolate high-quality genomic DNA from seed tissues streamlines the workflow to obtain direct estimates of seed dispersal distances in gymnosperms |
Autor: | García, C; Escribano-Ávila, Gema CSIC ORCID | Palabras clave: | Maternal tree Allele matching Embryo Endocarp Megagametophyte SSRs Maternity analyses |
Fecha de publicación: | 4-mar-2016 | Editor: | Springer Nature | Citación: | Journal of Plant Research 129(3): 559-563 (2016) | Resumen: | Genotyping of maternally derived seed tissues from georefered seeds that moved away from their source tree yield direct estimates of seed dispersal distances when the location and the genotype of the fruiting tree are available. These estimates are instrumental in forecasting the response of plant communities to drivers of global change, such as fragmentation or the expansion of invasive species. Obtaining robust assessments of seed dispersal distances requires comparing reliable multilocus genotypes of maternally derived seed tissues and fruiting trees, as previously shown for angiosperm species. However, robust estimates of seed dispersal distances based on direct methods are rare in non-model gymnosperms due to the difficulty in isolating high quality DNA from inconspicuous maternally derived seed tissues. These tissues tend to yield low DNA quantities that increase the frequency of genotyping errors. Here, we deliver a step-by-step visual protocol used to identify and isolate different seed tissues of interest for dispersal studies: embryos (2n, bi-parentally derived), seed coats (2n, maternally derived), and megagametophytes (n, maternally derived). We also provide an optimised lab protocol used to obtain multilocus genotypes from the target seed tissue. These broadly applicable protocols proved successful both in avoiding contamination among different seed tissues and providing reliable multilocus genotypes. | Versión del editor: | https://doi.org/10.1007/s10265-016-0806-3 | URI: | http://hdl.handle.net/10261/151719 | DOI: | 10.1007/s10265-016-0806-3 | Identificadores: | doi: 10.1007/s10265-016-0806-3 issn: 0918-9440 |
Aparece en las colecciones: | (IMEDEA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
2
checked on 18-abr-2024
WEB OF SCIENCETM
Citations
1
checked on 28-feb-2024
Page view(s)
198
checked on 19-abr-2024
Download(s)
45
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.