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Título: | Third Generation Antivenomics: Pushing the Limits of the In Vitro Preclinical Assessment of Antivenoms. |
Autor: | Pla, Davinia CSIC ORCID ; Rodríguez, Yania CSIC; Calvete, Juan J. CSIC ORCID | Palabras clave: | Snake venom Antivenoms Preclinical assessment of antivenom Third generation antivenomics Size-exclusion analysis of IgG-toxin complexes |
Fecha de publicación: | 10-may-2017 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Toxins 9(5): 158 (2017) | Resumen: | Second generation antivenomics is a translational venomics approach designed to complement in vivo preclinical neutralization assays. It provides qualitative and quantitative information on the set of homologous and heterologous venom proteins presenting antivenom-recognized epitopes and those exhibiting impaired immunoreactivity. In a situation of worrying antivenom shortage in many tropical and sub-tropical regions with high snakebite mortality and morbidity rates, such knowledge has the potential to facilitate the optimal deployment of currently existing antivenoms and to aid in the rational design of novel broad specificity antidotes. The aim of the present work was to expand the analytical capability of the immunoaffinity second-generation antivenomics platform, endowing it with the ability to determine the maximal binding capacity of an antivenom toward the different toxins present in a venom, and to quantify the fraction of venom-specific antibodies present in a given antivenom. The application of this new platform, termed third generation (3G) antivenomics, in the preclinical evaluation of antivenoms is illustrated in this paper for the case of antivenom EchiTAb-Plus-ICP® reactivity towards the toxins of homologous (B. arietans) and heterologous (N. melanoleuca) venoms. | Descripción: | 12 páginas, 5 figuras. | Versión del editor: | http://dx.doi.org/10.3390/toxins9050158 | URI: | http://hdl.handle.net/10261/151282 | DOI: | 10.3390/toxins9050158. | E-ISSN: | 2072-6651 |
Aparece en las colecciones: | (IBV) Artículos |
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2017 toxins-09-00158.pdf | 2,35 MB | Adobe PDF | Visualizar/Abrir |
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