Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/150471
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Combined epigenetic and intraspecific variation of the DRD4 and SERT genes influence novelty seeking behavior in great tit Parus major |
Autor: | Riyahi, Sepand; Sánchez-Delgado, Marta; Calafell, Francesc CSIC ORCID ; Monk, David; Senar, Juan Carlos CSIC ORCID | Palabras clave: | DRD4 Methylation Novelty seeking Personality traits SERT Urbanization |
Fecha de publicación: | 2015 | Editor: | Taylor & Francis | Citación: | Epigenetics 10(6): 516-525 (2015) | Resumen: | DNA methylation is one of the main epigenetic mechanisms that can regulate gene expression and is an important means for creating phenotypic variation. In the present study, we performed methylation profiling of 2 candidate genes for personality traits, namely DRD4 and SERT, in the great tit Parus major to ascertain whether personality traits and behavior within different habitats have evolved with the aid of epigenetic variation. We applied bisulphite PCR and strand-specific sequencing to determine the methylation profile of the CpG dinucleotides in the DRD4 and SERT promoters and also in the CpG island overlapping DRD4 exon 3. Furthermore, we performed pyrosequencing to quantify the total methylation levels at each CpG location. Our results indicated that methylation was ∼1–4% higher in urban than in forest birds, for all loci and tissues analyzed, suggesting that this epigenetic modification is influenced by environmental conditions. Screening of genomic DNA sequence revealed that the SERT promoter is CpG poor region. The methylation at a single CpG dinucleotide located 288 bp from the transcription start site was related to exploration score in urban birds. In addition, the genotypes of the SERT polymorphism SNP234 located within the minimal promoter were significantly correlated with novelty seeking behavior in captivity, with the allele increasing this behavior being more frequent in urban birds. As a conclusion, it seems that both genetic and methylation variability of the SERT gene have an important role in shaping personality traits in great tits, whereas genetic and methylation variation at the DRD4 gene is not strongly involved in behavior and personality traits. | Versión del editor: | http://dx.doi.org/10.1080/15592294.2015.1046027 | URI: | http://hdl.handle.net/10261/150471 | DOI: | 10.1080/15592294.2015.1046027 | ISSN: | 1559-2294 | E-ISSN: | 1559-2308 |
Aparece en las colecciones: | (IBE) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
24
checked on 21-abr-2024
SCOPUSTM
Citations
61
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
56
checked on 25-feb-2024
Page view(s)
263
checked on 24-abr-2024
Download(s)
77
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.