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Título: | Identification of antioxidant peptides of hen egg-white lysozyme and evaluation of inhibition of lipid peroxidation and cytotoxicity in the Zebrafish model |
Autor: | Carrillo, W. CSIC ORCID; Gómez-Ruiz, José Ángel CSIC; Miralles, Beatriz CSIC ORCID ; Ramos González, María Mercedes CSIC ; Recio, Isidra CSIC ORCID | Palabras clave: | Lysozyme Bioactive peptides Hydrolyzate Antioxidant activity in Zebrafish larvae Toxicity in Zebrafish egg Cation-exchange column |
Fecha de publicación: | 2016 | Editor: | Springer Nature | Citación: | European Food Research and Technology 242(10): 1777-1785 (2016) | Resumen: | Hen egg lysozyme was hydrolyzed with pepsin in situ on a cation-exchange column to isolate antioxidant peptides. The most cationic fraction was eluted with 1 M NaCl. Five positively charged peptides f(109–119) VAWRNRCKGTD, f(111–119) WRNRCKGTD, f(122–129) AWIRGCRL, f(123–129) WIRGCRL and f(124–129) IRGCRL were identified using tandem mass spectrometry. Using ORAC-FL , all five peptides presented antioxidant activity with values of (1970, 3123, 2743, 2393 and 0.313 µmol Trolox/µmol peptide), respectively. Using method TBARS in Zebrafish larvae, all five synthetic peptides were found to efficiently inhibit lipid peroxidation (36.8, 51.6, 55.56, 63.2, 61.0 % inhibition of lipid peroxidation), respectively. None of the five peptides were toxic in Zebrafish eggs and larvae at concentrations lower than 50 µg/ml. Concentrations higher than 50 µg/ml were toxic for both Zebrafish eggs and larvae. | Versión del editor: | https://doi.org/10.1007/s00217-016-2677-1 | URI: | http://hdl.handle.net/10261/150277 | DOI: | 10.1007/s00217-016-2677-1 | Identificadores: | doi: 10.1007/s00217-016-2677-1 e-issn: 1438-2385 issn: 1438-2377 |
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