English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/149220
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Effect of Different Broad Waveband Lights on Membrane Lipids of a Cyanobacterium, Synechococcus sp., as Determined by UPLC-QToF-MS and Vibrational Spectroscopy

AuthorsMontero, Olimpio; Velasco, Marta; Sanz-Arranz, Aurelio; Rull, Fernando
KeywordsSynechococcus sp.
UPLC-MS
Vibrational spectroscopy
Raman
IR
Lipids
Pigments
Light quality
Issue Date23-May-2016
PublisherMultidisciplinary Digital Publishing Institute
CitationBiology 5(2): 22 (2016)
AbstractDifferential profile of membrane lipids and pigments of a Synechococcus sp. cyanobacterial strain cells exposed to blue, green, red and white light are determined by means of liquid chromatography and mass spectrometry or diode array detection. Raman and ATR-IR spectra of intact cells under the diverse light wavebands are also reported. Blue light cells exhibited an increased content of photosynthetic pigments as well as specific species of membrane glycerolipids as compared to cells exposed to other wavebands. The A630/A680 ratio indicated an increased content of phycobilisomes (PBS) in the blue light-exposed cells. Some differences in the protein conformation between the four light waveband-exposed cells were deduced from the variable absorbance at specific wavenumbers in the FT-Raman and ATR-FTIR spectra, in particular bands assigned to amide I and amide II. Bands from 1180 to 950 cm´1 in the ATR-FTIR spectrum suggest degraded outer membrane polysaccharide in the blue light-exposed cells.
Publisher version (URL)http://doi.org/10.3390/biology5020022
URIhttp://hdl.handle.net/10261/149220
DOI10.3390/biology5020022
E-ISSN2079-7737
Appears in Collections:(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
biology-05-00022.pdf2,64 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.