Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/148662
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Interleukin-13 immune gene therapy prevents CNS inflammation and demyelination via alternative activation of microglia and macrophages |
Autor: | Guglielmetti, C.; Salas-Perdomo, Angélica CSIC ORCID; Planas, Anna M. CSIC ORCID; Ponsaerts, Peter | Palabras clave: | Magnetic resonance imaging Multiple sclerosis Demyelination |
Fecha de publicación: | dic-2016 | Editor: | John Wiley & Sons | Citación: | Glia 64(12): 2181-2200 (2016) | Resumen: | Detrimental inflammatory responses in the central nervous system are a hallmark of various brain injuries and diseases. With this study we provide evidence that lentiviral vector-mediated expression of the immune-modulating cytokine interleukin 13 (IL-13) induces an alternative activation program in both microglia and macrophages conferring protection against severe oligodendrocyte loss and demyelination in the cuprizone mouse model for multiple sclerosis (MS). First, IL-13 mediated modulation of cuprizone induced lesions was monitored using T-weighted magnetic resonance imaging and magnetization transfer imaging, and further correlated with quantitative histological analyses for inflammatory cell influx, oligodendrocyte death, and demyelination. Second, following IL-13 immune gene therapy in cuprizone-treated eGFP bone marrow chimeric mice, we provide evidence that IL-13 directs the polarization of both brain-resident microglia and infiltrating macrophages towards an alternatively activated phenotype, thereby promoting the conversion of a pro-inflammatory environment toward an anti-inflammatory environment, as further evidenced by gene expression analyses. Finally, we show that IL-13 immune gene therapy is also able to limit lesion severity in a pre-existing inflammatory environment. In conclusion, these results highlight the potential of IL-13 to modulate microglia/macrophage responses and to improve disease outcome in a mouse model for MS. GLIA 2016;64:2181–2200. | Versión del editor: | https://doi.org/10.1002/glia.23053 | URI: | http://hdl.handle.net/10261/148662 | DOI: | 10.1002/glia.23053 | Identificadores: | doi: 10.1002/glia.23053 issn: 1098-1136 |
Aparece en las colecciones: | (IIBB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
53
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
50
checked on 24-feb-2024
Page view(s)
238
checked on 16-abr-2024
Download(s)
173
checked on 16-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.