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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/14810
Title: Synthesis of overlapping fibrin citrullinated peptides and their use for diagnosing rheumatoid arthritis
Authors: Pérez Rodríguez, María L.; Gómara Elena, María José; Kasi, Devi; Alonso, Ana; Viñas, O.; Ercilla, Guadalupe; Sanmartí, Raimon; Haro Villar, Isabel
Keywords: Citrulline
Enzyme-linked immunosorbent assay
Rheumatoid arthritis
Synthetic peptides
Issue Date: Oct-2006
Publisher: Blackwell Publishing
Citation: Chemical Biology and Drug Design 68(4): 194-200 (2006)
Abstract: With the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to α- and β-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit621,627,630]α-fibrin(617–631) (αfib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.
Description: 7 pages, 2 figures.-- PMID: 17105483 [PubMed].
Publisher version (URL): http://dx.doi.org/10.1111/j.1747-0285.2006.00438.x
URI: http://hdl.handle.net/10261/14810
DOI: 10.1111/j.1747-0285.2006.00438.x
ISSN: 1747-0277 (Print)
1747-0285 (Online)
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