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Título : Synthesis of overlapping fibrin citrullinated peptides and their use for diagnosing rheumatoid arthritis
Autor : Pérez Rodríguez, María L., Gómara Elena, María José, Kasi, Devi, Alonso, Ana, Viñas, O., Ercilla, Guadalupe, Sanmartí, Raimon, Haro Villar, Isabel
Palabras clave : Citrulline
Enzyme-linked immunosorbent assay
Rheumatoid arthritis
Synthetic peptides
Fecha de publicación : Oct-2006
Editor: Blackwell Publishing
Resumen: With the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to α- and β-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit621,627,630]α-fibrin(617–631) (αfib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.
Descripción : 7 pages, 2 figures.-- PMID: 17105483 [PubMed].
Versión del editor:
ISSN: 1747-0277 (Print)
1747-0285 (Online)
DOI: 10.1111/j.1747-0285.2006.00438.x
Citación : Chemical Biology and Drug Design 68(4): 194-200 (2006)
Appears in Collections:(IQAC) Artículos

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