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Closed Access item Synthesis of overlapping fibrin citrullinated peptides and their use for diagnosing rheumatoid arthritis

Authors:Pérez Rodríguez, María L.
Gómara Elena, María José
Kasi, Devi
Alonso, Ana
Viñas, O.
Ercilla, Guadalupe
Sanmartí, Raimon
Haro Villar, Isabel
Keywords:Citrulline, Diagnosis, Enzyme-linked immunosorbent assay, Fibrin, Rheumatoid arthritis, Synthetic peptides
Issue Date:Oct-2006
Publisher:Blackwell Publishing
Citation:Chemical Biology and Drug Design 68(4): 194-200 (2006)
Abstract:With the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to α- and β-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit621,627,630]α-fibrin(617–631) (αfib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.
Description:7 pages, 2 figures.-- PMID: 17105483 [PubMed].
Publisher version (URL):http://dx.doi.org/10.1111/j.1747-0285.2006.00438.x
ISSN:1747-0277 (Print)
1747-0285 (Online)
Appears in Collections:(IQAC) Artículos

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