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Quantitative RT-PCR Platform for Transcript Profiling of C-N Metabolism Related Genes in Durum Wheat: Study under a Future Climate Change Scenario

AuthorsMorcuende, Rosa ; Vicente, Rubén; Martínez-Carrasco, Rafael ; Usadel, Björn; Kostadinova, Svetla; Pérez Pérez, Pilar
Issue DateApr-2016
AbstractClimate change is a major challenge to global food supply, thus is important understanding the mechanisms of crop responses to future environmental conditions. To achieve this goal, we developed a qRT-PCR platform for the expression analysis of more than a hundred C and N metabolism genes in durum wheat, based on available bread wheat genes and the identification of orthologs of known genes in other species. Additionally, we investigated the effect of elevated CO2 and temperature on primary metabolism of durum wheat grown in field chambers at two levels of N supply by combining transcript level analysis, using the qRT-PCR platform, with biochemical and physiological parameters in flag leaves at anthesis.
Description4 figuras. -- Póster presentado en el congreso: BIT's 6th Annual World Congress of Molecular & Cell Biology 2016. Theme: Unlocking the Secrets of Cells. Dalian (China), 25-28 de abril de 2016.
Appears in Collections:(IRNASA) Comunicaciones congresos
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