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Title

Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV-C/HGV

AuthorsPérez, Teresa; Ercilla, Guadalupe; Chan, Weng C.; Haro Villar, Isabel
KeywordsGBV-C/HGV
Diagnosis
Solid-phase peptide synthesis
Chimeric peptides
Cyclic peptides
ELISA
Circular dichroism
Fourier transformed infrared spectroscopy
Issue DateApr-2006
PublisherEuropean Peptide Society
John Wiley & Sons
CitationJournal of Peptide Science 12(4): 267-278 (2006)
AbstractIn this work, new putative epitopes located in nonstructural proteins of GBV-C/HGV were synthesized using solid-phase chemistry for their use in immunoassays. The antigens were obtained in linear, chimeric and cyclic forms with the main aim of improving the sensitivity of the enzyme immunoassays. Our results showed, on one hand, that the combination of different antigens seems to be necessary to ensure good sensitivity and more specificity and, on the other hand, that cyclic compounds show higher ability to recognize anti-GBV-C/HGV antibodies than its parent peptide. Furthermore, CD and FTIR have been used in conjunction to characterize the conformational changes therein with synthetic constructs that could explain their different antigenicity.
Description12 pages, 7 figures.-- PMID: 16180243 [PubMed].-- Available online Sep 23, 2005.
Publisher version (URL)http://dx.doi.org/10.1002/psc.719
URIhttp://hdl.handle.net/10261/14706
DOI10.1002/psc.719
ISSN1075-2617
E-ISSN1099-1387
Appears in Collections:(IQAC) Artículos
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