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A new real-time PCR method for rapid and specific detection of Ling (Molva molva)

AuthorsTaboada, Lucía; Sánchez, Ana Cristina ; González Sotelo, Carmen
KeywordsSeafood authentication
Real-time PCR
Molva molva
Issue Date2017
CitationFood Chemistry 228: 469-475 (2017)
AbstractSeafood fraud – often involving substitution of one species by another – has attracted much attention as it is prevalent worldwide. Whilst DNA analysis has helped to combat this type of fraud some of the methods currently in use are time-consuming and require sophisticated equipment or highly-trained personnel. This work describes the development of a new, real-time PCR TaqMan assay for the detection of ling (Molva molva) in seafood products. For this purpose, specific primers and a minor groove binding (MGB) TaqMan probe were designed to amplify the 81 bp region on the cyt b gene. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct value obtained for Molva molva DNA (19.45 ± 0.65) and the average Ct for non-target species DNA (38.3 ± 2.8), even with closely related species such as Molva dypterygia (34.9 ± 0.09). The proposed methodology has been validated with 31 commercial samples
Description7 páginas, 3 figuras, 3 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.foodchem.2017.01.117
Appears in Collections:(IIM) Artículos
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