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http://hdl.handle.net/10261/145679
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Higuera, José J. | es_ES |
dc.contributor.author | Calatrava, Victoria | es_ES |
dc.contributor.author | González, Zaira | es_ES |
dc.contributor.author | Mariscal, Vicente | es_ES |
dc.contributor.author | Siveiro, José M. | es_ES |
dc.contributor.author | Fernández, Emilio | es_ES |
dc.contributor.author | Galván, Aurora | es_ES |
dc.date.accessioned | 2017-02-24T07:10:15Z | - |
dc.date.available | 2017-02-24T07:10:15Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Agronomy 6 (1): 20 (2016) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/145679 | - |
dc.description.abstract | The NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Dynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT. | es_ES |
dc.description.sponsorship | We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). | - |
dc.description.sponsorship | This work was funded by MINECO (Ministerio de Economía y Competitividad, Spain, Grant No. BFU2011-29338) with support of Fondo Europeo de Desarrollo Regional (FEDER) program, Junta de Andalucía (P08-CVI-04157, BIO-128 and BIO-502), and Plan Propio de la Universidad de Córdoba. Authors thank Maribel Macías for skillful technical assistance. We thank the “Servicio Central de Apoyo a la Investigación” (SCAI, University of Córdoba, Spain) for DNA sequencing. | - |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | - |
dc.subject | Half-size transporter | es_ES |
dc.subject | Nitrite transport | es_ES |
dc.subject | Gene expression | es_ES |
dc.subject | Hansenula complementation | es_ES |
dc.title | NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family | es_ES |
dc.type | artículo | - |
dc.identifier.doi | 10.3390/agronomy6010020 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.3390/agronomy6010020 | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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agronomy-06-00020.pdf | 2,37 MB | Adobe PDF | Visualizar/Abrir |
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