Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/145679
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorHiguera, José J.es_ES
dc.contributor.authorCalatrava, Victoriaes_ES
dc.contributor.authorGonzález, Zairaes_ES
dc.contributor.authorMariscal, Vicentees_ES
dc.contributor.authorSiveiro, José M.es_ES
dc.contributor.authorFernández, Emilioes_ES
dc.contributor.authorGalván, Auroraes_ES
dc.date.accessioned2017-02-24T07:10:15Z-
dc.date.available2017-02-24T07:10:15Z-
dc.date.issued2016-
dc.identifier.citationAgronomy 6 (1): 20 (2016)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/145679-
dc.description.abstractThe NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Dynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT.es_ES
dc.description.sponsorshipWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).-
dc.description.sponsorshipThis work was funded by MINECO (Ministerio de Economía y Competitividad, Spain, Grant No. BFU2011-29338) with support of Fondo Europeo de Desarrollo Regional (FEDER) program, Junta de Andalucía (P08-CVI-04157, BIO-128 and BIO-502), and Plan Propio de la Universidad de Córdoba. Authors thank Maribel Macías for skillful technical assistance. We thank the “Servicio Central de Apoyo a la Investigación” (SCAI, University of Córdoba, Spain) for DNA sequencing.-
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institutees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccess-
dc.subjectHalf-size transporteres_ES
dc.subjectNitrite transportes_ES
dc.subjectGene expressiones_ES
dc.subjectHansenula complementationes_ES
dc.titleNRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Familyes_ES
dc.typeartículo-
dc.identifier.doi10.3390/agronomy6010020-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/agronomy6010020es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderJunta de Andalucía-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011011es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
Aparece en las colecciones: (IBVF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
agronomy-06-00020.pdf2,37 MBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

8
checked on 28-mar-2024

WEB OF SCIENCETM
Citations

6
checked on 28-feb-2024

Page view(s)

464
checked on 28-mar-2024

Download(s)

237
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.