English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/145679
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family

AuthorsHiguera, José J.; Calatrava, Victoria; González, Zaira; Mariscal, Vicente ; Siveiro, José M.; Fernández, Emilio; Galván, Aurora
KeywordsHalf-size transporter
Nitrite transport
Gene expression
Hansenula complementation
Issue Date2016
PublisherMultidisciplinary Digital Publishing Institute
CitationAgronomy 6 (1): 20 (2016)
AbstractThe NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Dynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT.
Publisher version (URL)http://dx.doi.org/10.3390/agronomy6010020
URIhttp://hdl.handle.net/10261/145679
DOI10.3390/agronomy6010020
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
agronomy-06-00020.pdf2,37 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.