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http://hdl.handle.net/10261/144219
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dc.contributor.author | Bermejo-Nogales, Azucena | - |
dc.contributor.author | Nederlof, Marit | - |
dc.contributor.author | Benedito-Palos, Laura | - |
dc.contributor.author | Ballester-Lozano, Gabriel F. | - |
dc.contributor.author | Folkedal, Ole | - |
dc.contributor.author | Olsen, Rolf Eric | - |
dc.contributor.author | Sitjà-Bobadilla, Ariadna | - |
dc.contributor.author | Pérez-Sánchez, Jaume | - |
dc.date.accessioned | 2017-02-17T12:59:42Z | - |
dc.date.available | 2017-02-17T12:59:42Z | - |
dc.date.issued | 2014-09-01 | - |
dc.identifier | issn: 1095-6840 | - |
dc.identifier.citation | General and Comparative Endocrinology 205: 305-315 (2014) | - |
dc.identifier.uri | http://hdl.handle.net/10261/144219 | - |
dc.description.abstract | The aim of the current study was to phenotype fish metabolism and the transcriptionally-mediated response of hepatic mitochondria of gilthead sea bream to intermittent and repetitive environmental stressors: (i) changes in water temperature (T-ST), (ii) changes in water level and chasing (C-ST) and (iii) multiple sensory perception stressors (M-ST). Gene expression profiling was done using a quantitative PCR array of 60 mitochondria-related genes, selected as markers of transcriptional regulation, oxidative metabolism, respiration uncoupling, antioxidant defense, protein import/folding/assembly, and mitochondrial dynamics and apoptosis. The mitochondrial phenotype mirrored changes in fish performance, haematology and lactate production. T-ST especially up-regulated transcriptional factors (PGC1α, NRF1, NRF2), rate limiting enzymes of fatty acid β-oxidation (CPT1A) and tricarboxylic acid cycle (CS), membrane translocases (Tim/TOM complex) and molecular chaperones (mtHsp10, mtHsp60, mtHsp70) to improve the oxidative capacity in a milieu of a reduced feed intake and impaired haematology. The lack of mitochondrial response, increased production of lactate and negligible effects on growth performance in C-ST fish were mostly considered as a switch from aerobic to anaerobic metabolism. A strong down-regulation of PGC1α, NRF1, NRF2, CPT1A, CS and markers of mitochondrial dynamics and apoptosis (BAX, BCLX, MFN2, MIRO2) occurred in M-ST fish in association with the greatest circulating cortisol concentration and a reduced lactate production and feed efficiency, which represents a metabolic condition with the highest allostatic load score. These findings evidence a high mitochondrial plasticity against stress stimuli, providing new insights to define the threshold level of stress condition in fish. © 2014 Elsevier Inc. | - |
dc.description.sponsorship | This work was funded by the EU AQUAEXCEL (Aquaculture Infrastructures for Excellence in European Fish Research, FP7/2007/2013; grant agreement No. 262336), and the Spanish AQUAGENOMICS (CSD2007-00002, Improvement of aquaculture production by the use of biotechnological tools) projects. Additional funding was obtained by Generalitat Valenciana (research grant PROMETEO 2010/006). | - |
dc.publisher | Elsevier | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/262336 | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.subject | Mitochondrial metabolism | - |
dc.subject | Thermal stress | - |
dc.subject | Teleost | - |
dc.subject | Husbandry stress | - |
dc.title | Metabolic and transcriptional responses of gilthead sea bream (Sparus aurata L.) to environmental stress: New insights in fish mitochondrial phenotyping | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.ygcen.2014.04.016 | - |
dc.relation.publisherversion | https://doi.org/10.1016/j.ygcen.2014.04.016 | - |
dc.date.updated | 2017-02-17T12:59:43Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Generalitat Valenciana | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | European Commission | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003359 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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2014-Metabolic and transcriptional responses of gilthead sea bream.pdf | 1,16 MB | Adobe PDF | Visualizar/Abrir |
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