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dc.contributor.authorBermejo-Nogales, Azucena-
dc.contributor.authorNederlof, Marit-
dc.contributor.authorBenedito-Palos, Laura-
dc.contributor.authorBallester-Lozano, Gabriel F.-
dc.contributor.authorFolkedal, Ole-
dc.contributor.authorOlsen, Rolf Eric-
dc.contributor.authorSitjà-Bobadilla, Ariadna-
dc.contributor.authorPérez-Sánchez, Jaume-
dc.date.accessioned2017-02-17T12:59:42Z-
dc.date.available2017-02-17T12:59:42Z-
dc.date.issued2014-09-01-
dc.identifierissn: 1095-6840-
dc.identifier.citationGeneral and Comparative Endocrinology 205: 305-315 (2014)-
dc.identifier.urihttp://hdl.handle.net/10261/144219-
dc.description.abstractThe aim of the current study was to phenotype fish metabolism and the transcriptionally-mediated response of hepatic mitochondria of gilthead sea bream to intermittent and repetitive environmental stressors: (i) changes in water temperature (T-ST), (ii) changes in water level and chasing (C-ST) and (iii) multiple sensory perception stressors (M-ST). Gene expression profiling was done using a quantitative PCR array of 60 mitochondria-related genes, selected as markers of transcriptional regulation, oxidative metabolism, respiration uncoupling, antioxidant defense, protein import/folding/assembly, and mitochondrial dynamics and apoptosis. The mitochondrial phenotype mirrored changes in fish performance, haematology and lactate production. T-ST especially up-regulated transcriptional factors (PGC1α, NRF1, NRF2), rate limiting enzymes of fatty acid β-oxidation (CPT1A) and tricarboxylic acid cycle (CS), membrane translocases (Tim/TOM complex) and molecular chaperones (mtHsp10, mtHsp60, mtHsp70) to improve the oxidative capacity in a milieu of a reduced feed intake and impaired haematology. The lack of mitochondrial response, increased production of lactate and negligible effects on growth performance in C-ST fish were mostly considered as a switch from aerobic to anaerobic metabolism. A strong down-regulation of PGC1α, NRF1, NRF2, CPT1A, CS and markers of mitochondrial dynamics and apoptosis (BAX, BCLX, MFN2, MIRO2) occurred in M-ST fish in association with the greatest circulating cortisol concentration and a reduced lactate production and feed efficiency, which represents a metabolic condition with the highest allostatic load score. These findings evidence a high mitochondrial plasticity against stress stimuli, providing new insights to define the threshold level of stress condition in fish. © 2014 Elsevier Inc.-
dc.description.sponsorshipThis work was funded by the EU AQUAEXCEL (Aquaculture Infrastructures for Excellence in European Fish Research, FP7/2007/2013; grant agreement No. 262336), and the Spanish AQUAGENOMICS (CSD2007-00002, Improvement of aquaculture production by the use of biotechnological tools) projects. Additional funding was obtained by Generalitat Valenciana (research grant PROMETEO 2010/006).-
dc.publisherElsevier-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/262336-
dc.relation.isversionofPostprint-
dc.rightsopenAccess-
dc.subjectMitochondrial metabolism-
dc.subjectThermal stress-
dc.subjectTeleost-
dc.subjectHusbandry stress-
dc.titleMetabolic and transcriptional responses of gilthead sea bream (Sparus aurata L.) to environmental stress: New insights in fish mitochondrial phenotyping-
dc.typeartículo-
dc.identifier.doi10.1016/j.ygcen.2014.04.016-
dc.relation.publisherversionhttps://doi.org/10.1016/j.ygcen.2014.04.016-
dc.date.updated2017-02-17T12:59:43Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderGeneralitat Valenciana-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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