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Bromodeoxyuridine dna labelling reveales the host intestinal proliferation induced by a myxozoan parasite and the time-space dependent proliferation of the parasitic stages

AuthorsPérez-Cordón, G. ; Estensoro, Itziar ; Sitjà-Bobadilla, Ariadna
Issue Date31-Aug-2015
Citation9th International Symposium on Fish Parasites (2015)
AbstractEnteromyxum leei is a myxozoan parasite responsible for enteritis in gilthead sea bream (GSB) (Sparus aurata). The parasite proliferates in the paracellular space and induces an inflammatory reaction. The infection starts at the posterior intestine (PI), followed by the anterior (AI) and finally the middle (MI). A group of juvenile GSB were infected by anal intubation with E. leei infected-intestinal scrapings (RCPT), and another was intubated with PBS (CTRL). At 17 and 64 days post intubation (dpi), 7 fish from both groups were intracelomically injected with 0.1 ml BrdU at a dose of 100 mg de BrdU/kg fish weight. Samples of AI, MI and PI were taken for immunohistochemical localization of BrdU 24 h after injection. For each fish and intestinal segment, ten microscopical fields were photographed and BdrU immunoreactive (BrdU+) cells (either from the parasite or the host) counted. There was a strong effect of the infection on the proliferation rate, as the number of BrdU+ cells was higher for RCPT than CTRL fish in all the intestinal segments. The presence of the parasite at the PI (the first segment infected) seems to induce the proliferation of host intestinal cells even in not yet parasitized segments (AI, MI). The mean ratio of BrdU+ parasites/total parasites in the PI was higher at 17 dpi (0.77) than at 64 dpi (0.30), whereas it was 0.58 and 0.61 at 64 dpi in the AI and MI, respectively. Therefore, the replicating rate of parasites in well-established infections is lower than that in recently invaded tissues. Acknowledgments: This work was funded by Spanish MINECO through projects AGL2009-13282 and AGL2013-48560 and partially supported by the ¿Generalitat Valenciana¿ (projects PROMETEOII/2014/085 and ISI).
DescriptionComunicación presentada en el 9th International Symposium on Fish Parasites, celebrado en Valencia, España, del 31 de agosto al 4 de septiembre de 2015
Appears in Collections:(IATS) Comunicaciones congresos
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