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Title

Absorption and distribution of ingested silica nanoparticles in Blattella germanica: a potential carrier in the development of nanopesticides

AuthorsBiagioni I. M.; Ochoa-Zapater M. A.; Ribera A.; Torreblanca, Amparo; Romero F. M.; Varó, Inmaculada
KeywordsIngestion
Nanopesticides
Silica-NPs
Blattella germanica
Issue Date3-May-2015
CitationSETAC Europe 25th Annual Meeting (2015)
AbstractThe rapid advances in nanotechnology have provided a wide range of nanomaterial with unique properties that open new possibilities of application in medicine, agricultural productivity and pest control. Nanoparticles can be used in agriculture as insecticide carriers in order to improve efficiency, reduce environmental pollution and the impact on non-target organisms of conventional pesticides. Mesoporous silica nanoparticles could be promising candidates as carriers due to the tridimensional open network structure that allows to store and controllably release active agents. We investigate penetration, absorption and distribution of mesoporous silica NPs marked with the fluorophore rhodamine B in the digestive tract of the model insect Blattella germanica. Silica NPs administration was carried out through food and insects were frozen at different exposure times at -80oC. Finally, insects were dissected and analyzed using an Olympus FV1000 fluorescence confocal microscope with 10x and 60x objectives. It was confirmed the presence of silica NPs in the alimentary canal. Furthermore NPs were observed in the enteric caeca suggesting that silica nanoparticles could cross the peritrophic membrane in Blattella germanica. It is likely the presence of silica NPS in other organs although it has not been confirmed yet.
DescriptionComunicación presentada en la SETAC Europe 25th Annual Meeting, celebrada en Barcelona, España, del 3 al 7 de mayo de 2015
URIhttp://hdl.handle.net/10261/143858
Appears in Collections:(IATS) Comunicaciones congresos
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