English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/143516
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

MHC class II B diversity in blue tits: a preliminary study

AuthorsRivero de Aguilar, Juan ; Schut, Elske; Merino, Santiago ; Martínez, Javier ; Komdeur, Jan; Westerdahl, Helena
KeywordsCyanistes caeruleus
Parus caeruleus
Passerine
Major histocompatibility complex
Issue Date2013
PublisherJohn Wiley & Sons
European Society of Evolutionary Biology
Society for the Study of Evolution
CitationEcology and Evolution 3(7): 1878-1889 (2013)
AbstractIn this study, we partly characterize major histocompatibility complex (MHC) class II B in the blue tit (Cyanistes caeruleus). A total of 22 individuals from three different European locations: Spain, The Netherlands, and Sweden were screened for MHC allelic diversity. The MHC genes were investigated using both PCR-based methods and unamplified genomic DNA with restriction fragment length polymorphism (RFLP) and southern blots. A total of 13 different exon 2 sequences were obtained independently from DNA and/or RNA, thus confirming gene transcription and likely functionality of the genes. Nine out of 13 alleles were found in more than one country, and two alleles appeared in all countries. Positive selection was detected in the region coding for the peptide binding region (PBR). A maximum of three alleles per individual was detected by sequencing and the RFLP pattern consisted of 4-7 fragments, indicating a minimum number of 2-4 loci per individual. A phylogenetic analysis, demonstrated that the blue tit sequences are divergent compared to sequences from other passerines resembling a different MHC lineage than those possessed by most passerines studied to date. © 2013 The Authors. Ecology and Evolution.
URIhttp://hdl.handle.net/10261/143516
DOI10.1002/ece3.598
Identifiersdoi: 10.1002/ece3.598
issn: 2045-7758
Appears in Collections:(MNCN) Artículos
Files in This Item:
File Description SizeFormat 
EcolEvol 3(7) 1878-1889 (2013).pdf785,75 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.