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http://hdl.handle.net/10261/143346
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dc.contributor.author | Yu, Siming | - |
dc.contributor.author | Perálvarez-Marín, Alex | - |
dc.contributor.author | Minelli, Caterina | - |
dc.contributor.author | Faraudo, Jordi | - |
dc.contributor.author | Roig Serra, Anna | - |
dc.contributor.author | Laromaine, Anna | - |
dc.date.accessioned | 2017-02-02T12:12:17Z | - |
dc.date.available | 2017-02-02T12:12:17Z | - |
dc.date.issued | 2016-05-24 | - |
dc.identifier | issn: 2040-3372 | - |
dc.identifier.citation | Nanoscale 8: 14393- 14405 (2016) | - |
dc.identifier.uri | http://hdl.handle.net/10261/143346 | - |
dc.description.abstract | The variety of nanoparticles (NPs) used in biological applications is increasing and the study of their interaction with biological media is becoming more important. Proteins are commonly the first biomolecules that NPs encounter when they interact with biological systems either in vitro or in vivo. Among NPs, super-paramagnetic iron oxide nanoparticles (SPIONs) show great promise for medicine. In this work, we study in detail the formation, composition, and structure of a monolayer of bovine serum albumin (BSA) on SPIONs. We determine, both by molecular simulations and experimentally, that ten molecules of BSA form a monolayer around the outside of the SPIONs and their binding strength to the SPIONs is about 3.5 × 10−4 M, ten times higher than the adsorption of fetal bovine serum (FBS) on the same SPIONs. We elucidate a strong electrostatic interaction between BSA and the SPIONs, although the secondary structure of the protein is not affected. We present data that supports the strong binding of the BSA monolayer on SPIONs and the properties of the BSA layer as a protein-resistant coating. We believe that a complete understanding of the behavior and morphology of BSA-SPIONs and how the protein interacts with SPIONs is crucial for improving NP surface design and expanding the potential applications of SPIONs in nanomedicine. | - |
dc.description.sponsorship | The research leading to these results has received funding from; the People Program (Marie Curie Actions) of the EC 7th Framework Program under grant agreement no 303630 and co-funded by the European Social Fund, the Spanish Ministry of Economy MAT2015-64442-R, co-supported by FEDER funds, the Generalitat de Catalunya 2014SGR213, the COST Action MP1202, Severo Ochoa Program (SEV-2015-0496) co-funded by European Social Funds, Ramon y Cajal grant RYC-2010-06082 (AL) and the China Scholarship Council fellowship (SMY, 201206150053). CM thanks the Innovation R&D Programme of the UK National Measurement System. AP-M is a recipient of the Universitat Autònoma de Barcelona-Programa Banco de Santander Fellowship. We acknowledge the support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). | - |
dc.publisher | Royal Society of Chemistry (UK) | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/303630 | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Albumin-coated SPIONs: an experimental and theoretical evaluation of protein conformation, binding affinity and competition with serum proteins | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1039/c6nr01732k | - |
dc.relation.publisherversion | https://doi.org/10.1039/c6nr01732k | - |
dc.date.updated | 2017-02-02T12:12:19Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by-nc/3.0/ | - |
dc.contributor.funder | Generalitat de Catalunya | - |
dc.contributor.funder | Universidad Autónoma de Barcelona | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Banco Santander | - |
dc.contributor.funder | CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) | - |
dc.contributor.funder | China Scholarship Council | - |
dc.contributor.funder | European Commission | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002809 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004543 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011104 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100010784 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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