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Differential Modulation of igT and igM upon Parasitic, Bacterial, Viral, and Dietary challenges in a Perciform Fish

AuthorsPiazzon de Haro, María Carla ; Galindo-Villegas, Jorge; Pereiro, Patricia ; Estensoro, Itziar ; Calduch-Giner, Josep A. ; Gómez-Casado, Eduardo; Novoa, Beatriz ; Mulero, Victoriano; Sitjà-Bobadilla, Ariadna ; Pérez-Sánchez, Jaume
Sparus aurata
Infectious diseases
Issue Date27-Dec-2016
PublisherFrontiers Media
CitationFrontiers in Immunology 7: 637 (2016)
AbstractThree different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilthead sea bream IgM and IgT in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. Their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bacterial, parasitic, mucosal vaccination and dietary challenges are studied. GCB IgM and IgT have the prototypical structure when compared to other fish Igs. The constitutive expression of sIgM was the highest overall in all tissues, whereas mIgT expression was highest in mucosal tissues, such as gills and intestine. IgM and IgT were differentially regulated upon infection. IgT was highly upregulated locally upon infection with the intestinal parasite Enteromyxum leei or systemically after Nodavirus infection. Long-term intestinal parasitic infections increased the serum titer of both isotypes. Mucosal vaccination against Photobacterium damselae subsp. piscicida finely regulated the Ig response inducing a systemic increase of IgM titers in serum and a local IgT response in skin mucus when animals were exposed to the pathogen by bath challenge. Interestingly, plant-based diets inhibit IgT upregulation upon intestinal parasitic challenge, which was related to a worse disease outcome. All these results corroborate the mucosal role of IgT and emphasize the importance of a finely tuned regulation of Ig isotypes upon infection, which could be of special interest in vaccination studies
Description16 páginas, 8 figuras, 2 tablas.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
Publisher version (URL)http://dx.doi.org/10.3389/fimmu.2016.00637
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