Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/142755
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

A single mutation in the gene responsible for the mucoid phenotype of Bifidobacterium animalis subsp. lactis confers surface and functional characteristics

AutorHidalgo-Cantabrana, Claudio CSIC ORCID CVN; Sánchez García, Borja CSIC ORCID; Martínez Álvarez, Noelia CSIC ORCID; Ruas-Madiedo, Patricia CSIC ORCID ; Margolles Barros, Abelardo CSIC ORCID
Fecha de publicación11-sep-2015
EditorAmerican Society for Microbiology
CitaciónApplied and Environmental Microbiology 81(23): 7960-7968 (2015)
ResumenExopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of Bifidobacterium animalis subsp. lactis that differ in their EPS-producing phenotype. The original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. The sequence analysis of their genomes revealed a nonsynonymous mutation in the gene Balat_1410, putatively involved in the elongation of the EPS chain. By comparing a strain from which this gene had been deleted with strains containing the wild-type and mutated genes, we were able to show that each strain displays different cell surface characteristics. The mucoid EPS synthesized by the strain harboring the mutation in Balat_1410 provided higher resistance to gastrointestinal conditions and increased the capability for adhesion to human enterocytes. In addition, the cytokine profiles of human peripheral blood mononuclear cells and ex vivo colon tissues suggest that the mucoid strain could have higher anti-inflammatory activity. Our findings provide relevant data on the function of Balat_1410 and reveal that the mucoid phenotype is able to alter some of the most relevant functional properties of the cells.
Versión del editorhttps://doi.org/10.1128/AEM.02095-15
URIhttp://hdl.handle.net/10261/142755
DOI10.1128/AEM.02095-15
Identificadoresissn: 0099-2240
Aparece en las colecciones: (IPLA) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

18
checked on 17-mar-2024

SCOPUSTM   
Citations

37
checked on 13-mar-2024

WEB OF SCIENCETM
Citations

31
checked on 22-feb-2024

Page view(s)

295
checked on 19-mar-2024

Download(s)

87
checked on 19-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.