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A novel UHPLC method for the rapid and simultaneous determination of daidzein, genistein and equol in human urine

AuthorsRedruello, Begoña ; Guadamuro, Lucía ; Cuesta Suárez, Isabel ; Álvarez Buylla, J. R. ; Mayo Pérez, Baltasar ; Delgado, Susana
Menopausal women
Soy isoflavones
Human urine
Solid phase extraction
Issue Date15-Nov-2015
CitationJournal of chromatography. B, Analytical technologies in the biomedical and life sciences 1005: 1-8 (2015)
AbstractThis work reports on a novel method involving reverse-phased ultra-high performance liquid chromatography (UHPLC) plus a spectrophotometric photodiode array/fluorescence (FLR) detection system for determining the concentration of equol and major soy isoflavones (daidzein and genistein) in human urine. The proposed method was validated in terms of its linearity, sensitivity, accuracy (recovery) and precision (intra- and inter-day repeatability). The isoflavone profiles of urine samples from a group of menopausal women following oral soy isoflavone supplementation were determined and compared. Screening for equol-producer status was accomplished with high sensitivity (detection limit of the FLR detector 2.93 nM). The method involves a short chromatographic run time compared to conventional HPLC methods while allowing for the simultaneous and reliable quantification of daidzein, genistein and equol in human urine. It also allows for the rapid screening of multiple urine samples when testing for equol production status and checking patient adherence to isoflavone treatment regimens. © 2015 Elsevier B.V.
Publisher version (URL)https://doi.org/10.1016/j.jchromb.2015.09.029
Identifiersissn: 1873-376X
Appears in Collections:(IPLA) Artículos
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