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Título: | Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats |
Autor: | Almog, Mara; Korngreen, Alon | Palabras clave: | Voltage-Gated Ca2+ Pyramidal Neurons Rats Neocortex |
Fecha de publicación: | 1-abr-2009 | Editor: | Public Library of Science | Citación: | PLoS ONE. 2009; 4(4): e4841 | Resumen: | Neuronal voltage-gated Ca2+ channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca2+ channels is generating regenerative dendritic Ca2+ spikes. However, the Ca2+ dependent mechanisms used to create these spikes are only partially understood. To start investigating this mechanism, we set out to kinetically and pharmacologically identify the sub-types of somatic voltage-gated Ca2+ channels in pyramidal neurons from layer 5 of rat somatosensory cortex, using the nucleated configuration of the patch-clamp technique. The activation kinetics of the total Ba2+ current revealed conductance activation only at medium and high voltages suggesting that T-type calcium channels were not present in the patches. Steady-state inactivation protocols in combination with pharmacology revealed the expression of R-type channels. Furthermore, pharmacological experiments identified 5 voltage-gated Ca2+ channel sub-types – L-, N-, R- and P/Q-type. Finally, the activation of the Ca2+ conductances was examined using physiologically derived voltage-clamp protocols including a calcium spike protocol and a mock back-propagating action potential (mBPAP) protocol. These experiments enable us to suggest the possible contribution of the five Ca2+ channel sub-types to Ca2+ current flow during activation under physiological conditions. | Descripción: | 11 pages, 7 figures.-- PMID: 19337371 [PubMed].-- PMCID: PMC2659773. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0004841 | URI: | http://hdl.handle.net/10261/14207 | DOI: | 10.1371/journal.pone.0004841 | ISSN: | 1932-6203 |
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