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Título: | High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in a semi-extensive red deer (Cervus elaphus hispanicus) farm in Southern Spain |
Autor: | Gómez, Paula; Lozano, Carmen; González-Barrio, David CSIC ORCID; Zarazaga, Myriam; Ruiz-Fons, Francisco CSIC ORCID ; Torres, Carmen | Palabras clave: | CC130 IEC Red deer mecC MRSA S. aureus |
Fecha de publicación: | 2015 | Editor: | Elsevier | Citación: | Veterinary Microbiology 177(3-4): 326-331 (2015) | Resumen: | The objective was to determine the prevalence of Staphylococcus aureus nasal carriage in red deer of a semi-extensive farm and in humans in contact with the estate animals, and to characterize obtained isolates. Nasal swabs of 65 deer and 15 humans were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base. Isolates were identified by microbiological and molecular methods. Antimicrobial susceptibility profile was determined for 16 antibiotics by disk-diffusion and the presence of eight antibiotic resistance genes, seven virulence genes and genes of immune-evasion-cluster (IEC) was analyzed by PCR. S. aureus was typed by PFGE-SmaI, spa, agr, SCC. mec and MLST. Isolates were detected in 16 deer (24.6%). Eleven S. aureus isolates were methicillin-resistant (MRSA), and five were methicillin-susceptible (MSSA). All MRSA harbored mecC gene and were agr-III/SCC. mecXI/ST1945 (four spa-t843 and seven spa-t1535). All mecC-MRSA carried blaZ-SCC. mecXI and etd2, were IEC-type-E, and belonged to the same PFGE pattern. The five MSSA were typed as spa-t2420/. agr-I/ST133. Regarding humans, S. aureus was recovered from six samples (40%). The isolates were MSSA and were typed as spa-t002/. agr-II, spa-t012/. agr-III or spa-t822/. agr-III and showed different IEC types (A, B, D and F). blaZ and erm(A) genes were detected, as well as cna and tst genes. As conclusion, red deer analyzed in this study are frequent carriers of mecC-MRSA CC130 (16.9%), they are characterized by few resistance and virulence determinants, and by the presence of IEC type-E. Deer could be a source of mecC-MRSA which could potentially be transmitted to other animals, or even to humans. | URI: | http://hdl.handle.net/10261/142005 | DOI: | 10.1016/j.vetmic.2015.03.029 | Identificadores: | doi: 10.1016/j.vetmic.2015.03.029 e-issn: 1873-2542 issn: 0378-1135 |
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