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Título: | Inappropriate translation inhibition and P-body formation cause cold-sensitivity in tryptophan-auxotroph yeast mutants |
Autor: | Ballester Tomás, Lidia CSIC; Prieto Alamán, José Antonio CSIC ORCID; Alepuz, Paula; González, Asier; Garre, Elena CSIC; Rández Gil, Francisca CSIC ORCID | Palabras clave: | Yeast Low temperature Polysomes Gcn2 pathway eIF2α Hog1 Snf1 |
Fecha de publicación: | 15-nov-2016 | Editor: | Elsevier | Citación: | Biochimica et Biophysica Acta - Molecular Cell Research 1864 (2): 314-323 (2017) | Resumen: | In response to different adverse conditions, most eukaryotic organisms, including Saccharomyces cerevisiae, downregulate protein synthesis through the phosphorylation of eIF2α (eukaryotic initiation factor 2α) by Gcn2, a highly conserved protein kinase. Gcn2 also controls the translation of Gcn4, a transcription factor involved in the induction of amino acid biosynthesis enzymes. Here, we have studied the functional role of Gcn2 and Gcn2-regulating proteins, in controlling translation during temperature downshifts of TRP1 and trp1 yeast cells. Our results suggest that neither cold-instigated amino acid limitation nor Gcn2 are involved in the translation suppression at low temperature. However, loss of TRP1 causes increased eIF2α phosphorylation, Gcn2-dependent polysome disassembly and overactivity of Gcn4, which result in cold-sensitivity. Indeed, knock-out of GCN2 improves cold growth of trp1 cells. Likewise, mutation of several Gcn2-regulators and effectors results in cold-growth effects. Remarkably, we found that Hog1, the osmoresponsive MAPK, plays a role in the regulatory mechanism of Gcn2-eIF2α. Finally, we demonstrated that P-body formation responds to a downshift in temperature in a TRP1-dependent manner and is required for cold tolerance. | Versión del editor: | http://dx.doi.org/10.1016/j.bbamcr.2016.11.012 | URI: | http://hdl.handle.net/10261/141662 | DOI: | 10.1016/j.bbamcr.2016.11.012 | ISSN: | 1388-1981 |
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