Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/140539
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The cell division protein FtsZ from Streptococcus pneumoniae exhibits a GTPase activity delay |
Autor: | Salvarelli, Estefanía; Krupka, Marcin; Rivas, Germán CSIC ORCID CVN ; Mingnorance, Jesús; Gómez-Puertas, Paulino CSIC ORCID ; Alfonso, Carlos CSIC ORCID ; Rico, Ana Isabel | Fecha de publicación: | 1-sep-2015 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 290: 25081- 25089 (2015) | Resumen: | © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.. The cell division protein FtsZ assembles in vitro by a mechanism of cooperative association dependent on GTP, monovalent cations, and Mg2+. We have analyzed the GTPase activity and assembly dynamics of Streptococcus pneumoniae FtsZ (Spn-FtsZ). SpnFtsZ assembled in an apparently cooperative process, with a higher critical concentration than values reported for other FtsZ proteins. It sedimented in the presence of GTP as a high molecular mass polymer with a well defined size and tended to form double-stranded filaments in electron microscope preparations. GTPase activity depended on K+ and Mg2+ and was inhibited by Na+. GTP hydrolysis exhibited a delay that included a lag phase followed by a GTP hydrolysis activation step, until reaction reached the GTPase rate. The lag phase was not found in polymer assembly, suggesting a transition from an initial non-GTP-hydrolyzing polymer that switches to a GTP-hydrolyzing polymer, supporting models that explain FtsZ polymer cooperativity. | URI: | http://hdl.handle.net/10261/140539 | DOI: | 10.1074/jbc.M115.650077 | Identificadores: | doi: 10.1074/jbc.M115.650077 issn: 1083-351X |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Gomez-Puertas P The cell division.pdf | 1,86 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
6
checked on 08-abr-2024
SCOPUSTM
Citations
9
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
8
checked on 23-feb-2024
Page view(s)
170
checked on 24-abr-2024
Download(s)
210
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.