Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/14007
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Título : Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations
Autor : Canales García-Menocal, Mario, Almazán, Consuelo, Naranjo, María Victoria, Jongejan, Frans, Fuente García, José de la
Palabras clave : Boophilus annulatus
B. microplus
Bm86
Ba86
Recombinant
Vaccination
Fecha de publicación : 31-Mar-2009
Editor: BioMed Central
Citación : BMC Biotechnology 2009, 9:29
Resumen: [Background] The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick infestations. Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris.
[Results] Recombinant Ba86 (Israel strain) was used to immunize cattle to test its efficacy for the control of B. annulatus (Mercedes, Texas, USA strain) and B. microplus (Susceptible, Mexico strain) infestations. Bm86 (Gavac and Mozambique strain) and adjuvant/saline were used as positive and negative controls, respectively. Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively. The efficacy of both Ba86 and Bm86 was higher for B. annulatus than for B. microplus. The efficacy of Ba86 was higher for B. annulatus (83.0%) than for B. microplus (71.5%). The efficacy of Bm86 (Gavac; 85.2%) but not Bm86 (Mozambique strain; 70.4%) was higher than that of Ba86 (71.5%) on B. microplus. However, the efficacy of Bm86 (both Gavac and Mozambique strain; 99.6%) was higher than that of Ba86 (83.0%) on B. annulatus.
[Conclusion] These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.
Descripción : 8 pages,2 figures and 2 tables
Versión del editor: http://dx.doi.org/10.1186/1472-6750-9-29
URI : http://hdl.handle.net/10261/14007
ISSN: 1472-6750
DOI: 10.1186/1472-6750-9-29
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