Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/14007
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations

AutorCanales, Mario CSIC ; Almazán, Consuelo; Naranjo, María Victoria CSIC ; Jongejan, Frans; Fuente, José de la CSIC ORCID
Palabras claveBoophilus annulatus
B. microplus
Bm86
Ba86
Recombinant
Vaccination
Fecha de publicación31-mar-2009
EditorBioMed Central
CitaciónBMC Biotechnology 2009, 9:29
Resumen[Background] The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick infestations. Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris.
[Results] Recombinant Ba86 (Israel strain) was used to immunize cattle to test its efficacy for the control of B. annulatus (Mercedes, Texas, USA strain) and B. microplus (Susceptible, Mexico strain) infestations. Bm86 (Gavac and Mozambique strain) and adjuvant/saline were used as positive and negative controls, respectively. Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively. The efficacy of both Ba86 and Bm86 was higher for B. annulatus than for B. microplus. The efficacy of Ba86 was higher for B. annulatus (83.0%) than for B. microplus (71.5%). The efficacy of Bm86 (Gavac; 85.2%) but not Bm86 (Mozambique strain; 70.4%) was higher than that of Ba86 (71.5%) on B. microplus. However, the efficacy of Bm86 (both Gavac and Mozambique strain; 99.6%) was higher than that of Ba86 (83.0%) on B. annulatus.
[Conclusion] These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.
Descripción8 pages,2 figures and 2 tables
Versión del editorhttp://dx.doi.org/10.1186/1472-6750-9-29
URIhttp://hdl.handle.net/10261/14007
DOI10.1186/1472-6750-9-29
ISSN1472-6750
Aparece en las colecciones: (IREC) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
1472-6750-9-29.pdf258,37 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

31
checked on 21-feb-2024

SCOPUSTM   
Citations

99
checked on 15-mar-2024

WEB OF SCIENCETM
Citations

85
checked on 23-feb-2024

Page view(s)

436
checked on 18-mar-2024

Download(s)

243
checked on 18-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.