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Title

Subunit association as the stabilizing determinant for archaeal methionine adenosyltransferases

AuthorsGarrido, Francisco ; Alfonso, Carlos ; Taylor, John C.; Markham, George D.; Pajares, María A.
KeywordsS-adenosylmethionine synthetase
Tryptophan mutants
Methionine adenosyltransferase
Issue Date2009
PublisherElsevier
CitationBBA - Proteins and Proteomics 1794(7): 1082-1090 (2009)
AbstractArchaea contain a class of methionine adenosyltransferases (MATs) that exhibit substantially higher stability than their mesophilic counterparts. Their sequences are highly divergent, but preserve the essential active site motifs of the family. We have investigated the origin of this increased stability using chemical denaturation experiments on Methanococcus jannaschii MAT (Mj-MAT) and mutants containing single tryptophans in place of tyrosine residues. The results from fluorescence, circular dichroism, hydrodynamic, and enzyme activity measurements showed that the higher stability of Mj-MAT derives largely from a tighter association of its subunits in the dimer. Local fluorescence changes, interpreted using secondary structure predictions, further identify the least stable structural elements as the C-terminal ends of β-strands E2 and E6, and the N-terminus of E3. Dimer dissociation however requires a wider perturbation of the molecule. Additional analysis was initially hindered by the lack of crystal structures for archaeal MATs, a limitation that we overcame by construction of a 3D-homology model of Mj-MAT. This model predicts preservation of the chain topology and three-domain organization typical of this family, locates the least stable structural elements at the flat contact surface between monomers, and shows that alterations in all three domains are required for dimer dissociation.
DescriptionEl pdf del artículo es el manuscrito de autor.
Publisher version (URL)http://dx.doi.org/10.1016/j.bbapap.2009.03.018
URIhttp://hdl.handle.net/10261/13806
DOI10.1016/j.bbapap.2009.03.018
ISSN1570-9639
Appears in Collections:(IIBM) Artículos
(CIB) Artículos
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