English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/138052
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator

AuthorsDurante-Rodríguez, Gonzalo ; Mancheño, Jose M. ; Díaz, Eduardo ; Carmona, Manuel
KeywordsAzoarcus
Protein engineering
Synthetic biology
Transcriptional regulation
Issue DateAug-2016
PublisherJohn Wiley & Sons
CitationMicrobiologyOpen 5(4): 575–581 (2016)
AbstractIn this work, we explore the refactoring of the circuitry of λ phage by engineering a new-to-nature regulator that responds to an ad hoc input signal that behaves orthogonal with respect to the host cell. We tailored a chimeric regulator, termed Qλ, between the CI protein of the λ phage and the BzdR repressor from Azoarcus sp. strain CIB that responds to benzoyl-CoA. When the Qλ was expressed in the appropriate Escherichia coli cells, it was able to reprogram the lytic/lysogenic λ phage decision according to the intracellular production of benzoyl-CoA.Our results are also an example of how generating new artificial regulators that respond to effectors of choice may be useful to control different cellular processes.
Description7 p.-5 fig.-1 tab.
Publisher version (URL)http://dx.doi.org/ 10.1002/mbo3.352
URIhttp://hdl.handle.net/10261/138052
DOI10.1002/mbo3.352
E-ISSN2045-8827
Appears in Collections:(IQFR) Artículos
(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Durante-Rodr-guez_et_al-2016-MicrobiologyOpen.pdfArtículo principal244,48 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.