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Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator

AuthorsDurante-Rodríguez, Gonzalo ; Mancheño, Jose M. ; Díaz, Eduardo ; Carmona, Manuel
Protein engineering
Synthetic biology
Transcriptional regulation
Issue DateAug-2016
PublisherJohn Wiley & Sons
CitationMicrobiologyOpen 5(4): 575–581 (2016)
AbstractIn this work, we explore the refactoring of the circuitry of λ phage by engineering a new-to-nature regulator that responds to an ad hoc input signal that behaves orthogonal with respect to the host cell. We tailored a chimeric regulator, termed Qλ, between the CI protein of the λ phage and the BzdR repressor from Azoarcus sp. strain CIB that responds to benzoyl-CoA. When the Qλ was expressed in the appropriate Escherichia coli cells, it was able to reprogram the lytic/lysogenic λ phage decision according to the intracellular production of benzoyl-CoA.Our results are also an example of how generating new artificial regulators that respond to effectors of choice may be useful to control different cellular processes.
Description7 p.-5 fig.-1 tab.
Publisher version (URL)http://dx.doi.org/ 10.1002/mbo3.352
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