English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/136051
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Analysis of the interaction between desmosomal proteins: Desmoplakin and Plakophilin

AuthorsCarballido Vázquez, Ana M. ; Pereda, José M. de
Issue Date2012
Citation22 IUBMB (2012)
AbstractDesmosomes are intercellular junctions that anchor cytoskeletal intermediate filaments at the plasma membrane. This is achieved through a complex network of protein-protein interactions which provide a strong cell-cell adhesion and maintain the structural and functional integrity of tissues. Desmoplakin (DSP) and plakophilins (PKP) are components of these macromolecular complexes. DSP belongs to the plakin family of cytolinkers and contains in its N-terminal region a Plakin Domain –conserved in the whole family– that is formed by eight Spectrin Repeats and an SH3 domain. The Plakin Domain of DSP is important to localize DSP in the desmosomal plaque and to interact with binding partners such as PKPs, which are members of the armadillo protein family. We have designed a collection of DSP plakin domain and PKP1a fragments. The recombinants proteins were co-expressed in COS1 cells and the interactions were studied by co-immunoprecipitation. Our results suggest that the Plakin Domain of DSP harbors more than one interaction site for PKP1.
DescriptionResumen del póster presentado al 22nd IUBMB y 37th FEBS, celebrados en Sevilla (España) del 4 al 9 de septiembre de 2012.
URIhttp://hdl.handle.net/10261/136051
Appears in Collections:(IBMCC) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.