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Protist diversity assessment: Comparing microscopy, cytometry and molecular approaches

AutorFelip, Marisol; Triadó-Margarit, Xavier ; Casamayor, Emilio O. ; Gasol, Josep M. ; Catalán, Jordi
Fecha de publicación24-feb-2015
EditorAssociation for the Sciences of Limnology and Oceanography
Citación2015 Aquatic Sciences Meeting. Program Book: 94 (2015)
ResumenMicroscopic eukaryotes (protist) are an essential component of microbial food webs and have a key role in the functioning of planktonic systems. Protists have a high degree of structural and metabolic diversity. During the last years, the advent of cultivationindependent molecular methods has deeply influenced the assessment of aquatic microeukaryotic diversity formerly based exclusively on morphological taxonomy. However, comparative studies between molecular and morphological approaches in natural environments remain rare. Here, we evaluated the planktonic protists biodiversity in a set of 10 high mountain lakes of the Pyrenees using three approaches: morphological diversity, obtained by microscopic analysis; genetic diversity, based on cloning and sequencing of the 18S rRNA gene; and cytometric group diversity estimated from flow-cytometer data. Species richness and inverse Simpson index were compared using a range of similarity values to stablish phylotypes (OTUS) and several taxonomic classification levels to stablish morphotypes. Agreements and discrepancies between the three approaches for measuring protist diversity are discussed
DescripciónAquatic Sciences Meeting, Aquatic Sciences: Global And Regional Perspectives - North Meets South, 22-27 February 2015, Granada, Spain
Versión del editorhttp://www.sgmeet.com/aslo/granada2015/program.asp
Aparece en las colecciones: (ICM) Comunicaciones congresos
(CEAB) Comunicaciones congresos
(IDAEA) Comunicaciones congresos
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